The scientific, social, and economic advantages that accrue from Open Science (OS) practices—ways of doing research that emphasize reproducibility, transparency, and accessibility at all stages of the research cycle—are now widely recognized in nations around the world and by international bodies such as the United Nations and the Organization for Economic Cooperation and Development. However, program wide or coordinated instruction of undergraduate students in OS practices remains uncommon. At the University of British Columbia in Canada, we have started to develop a comprehensive undergraduate OS program that can be adapted to and woven into diverse subject curricula. We report on the context and planning of the pilot module of the program, “Open Science 101”, its implementation in first-year Biology in Fall 2019, and qualitative results of an attitudinal survey of students following their course.