Skip Nav Destination
Close Modal
Update search
NARROW
Format
Date
Availability
1-1 of 1
Mohamed N. M. Bahrudeen
Close
Follow your search
Access your saved searches in your account
Would you like to receive an alert when new items match your search?
Sort by
Proceedings Papers
. ecal2017, ECAL 2017, the Fourteenth European Conference on Artificial Life454-459, (September 4–8, 2017) doi: 10.1162/isal_a_075
Abstract
PDF
Measurements at the single cell level showed that monoclonal Escherichia coli cells differ widely in the numbers of components affecting gene expression dynamics. Using a stochastic model of a 2-genes symmetric toggle switch with realistic multi-step promoter initiation kinetics and empirically validated parameter values, we investigate the role of transcription initiation kinetics on the degree with which cell-to-cell variability in cellular components generates cell-to-cell diversity in switch dynamics. We find that while the mean switching frequency is determined by the promoter kinetics, the cell to cell diversity of this frequency depends both on promoter kinetics and diversity in RNA polymerase numbers. At a microscale level, the main regulator of the cell-to-cell variability in protein numbers (of both genes in ON and OFF states) is the promoters kinetics, not the diversity in RNA polymerase numbers. We conclude that the promoters kinetics is a critical regulator of the toggle switch dynamics and that can be used as a regulatable filter of extrinsic noise.