The study of bacterial art provides an opportunity to investigate the philosophical framework of object-oriented ontology (OOO). This viewpoint challenges traditional human-centered perspectives by highlighting the autonomy and agency of all objects, including microorganisms. Objects have their own inherent properties and are regarded as equal actors in the world. The authors highlight the dynamic relationship between humans and microorganisms and the agency of bacteria in bioart. Bacterial art raises concerns regarding control, authorship, aesthetics, and ethics, necessitating interdisciplinary discussions and critical evaluations. This study provides an intriguing case examining the implications of OOO at the intersection of science and art.