Recently, EEG recording techniques and source analysis have improved, making it feasible to tap into fast network dynamics. Yet, analyzing whole-cortex EEG signals in source space is not standard, partly because EEG suffers from volume conduction: Functional connectivity (FC) reflecting genuine functional relationships is impossible to disentangle from spurious FC introduced by volume conduction. Here, we investigate the relationship between white matter structural connectivity (SC) and large-scale network structure encoded in EEG-FC. We start by confirming that FC (power envelope correlations) is predicted by SC beyond the impact of Euclidean distance, in line with the assumption that SC mediates genuine FC. We then use information from white matter structural connectivity in order to smooth the EEG signal in the space spanned by graphs derived from SC. Thereby, FC between nearby, structurally connected brain regions increases while FC between nonconnected regions remains unchanged, resulting in an increase in genuine, SC-mediated FC. We analyze the induced changes in FC, assessing the resemblance between EEG-FC and volume-conduction- free fMRI-FC, and find that smoothing increases resemblance in terms of overall correlation and community structure. This result suggests that our method boosts genuine FC, an outcome that is of interest for many EEG network neuroscience questions.
In this study, we combine high-density EEG recorded during resting state with white matter connectivity obtained from diffusion MRI and fiber tracking. We leverage the additional information contained in the structural connectome towards augmenting the source-level EEG functional connectivity. In particular, it is known—and confirmed in this study—that the activity of brain regions that possess a direct anatomical connection is, on average, more strongly correlated than that of regions that have no such direct link. We use the structural connectome to define a graph and smooth the source-reconstructed EEG signal in the space spanned by this graph. We compare the resulting “filtered” signal correlation matrices with those obtained from fMRI and find that such “graph filtering” improves the agreement between EEG and fMRI functional connectivity structure. This suggests that structural connectivity can be used to attenuate some of the limitations imposed by volume conduction.
Electroencephalography (EEG)measures neural signals directly (Buzsáki, Anastassiou, & Koch, 2012) on a timescale of milliseconds, fast enough to be relevant for neural events. In MEG and fMRI, the study of functional connectivity (FC) with the tools of whole-brain network neuroscience has yielded a multitude of important and interesting insights (see Bassett & Sporns, 2017, for a review). Concurring findings show that FC between regions of interest (ROIs)/sources located in the gray matter is in part shaped by anatomical connections of the structural connectivity (SC; obtained from dMRI and fiber tracking), such that the strength of SC (fiber count, density) is predictive to some degree of the strength of FC (correlation, coherence, etc.; Abdelnour, Dayan, Devinsky, Thensen, & Raj, 2018; Atasoy, Donnelly, & Pearson, 2016; Cabral et al., 2014; Damoiseaux & Greicius, 2009; Deco et al., 2013; Glomb, Ponce-Alvarez, Gilson, Ritter, & Deco, 2017; Goñi et al., 2014; Hagmann et al., 2008; Honey et al., 2009; Meier et al., 2016; Tewarie et al., 2014, 2019; Vincent et al., 2007). This finding has been shown to extend to EEG data on the source level (i.e., signals recorded on the scalp projected into the gray matter) using analytical (Chu et al., 2015; Wirsich et al., 2017) and modeling approaches (Bhattacharya, Coyle, & Maguire, 2011; de Haan, Mott, van Straaten, Scheltens, & Stam, 2012; Finger et al., 2016; Pons, Cantero, Atienza, & Garcia-Ojalvo, 2010; Ponten, Daffertshofer, Hillebrand, & Stam, 2010; van Dellen et al., 2013). The main hurdle when trying to understand EEG network architecture and dynamics is signal leakage due to volume conduction, which obscures genuine functional relationships between sources in the brain: Additionally to the low spatial resolution (Buzsáki et al., 2012; Schoffelen & Gross, 2009; Srinivasan, Winter, Ding, & Nunez, 2007) and signal-to-noise ratio intrinsic to the EEG signal, the interaction of the electric field with the tissue creates “sham” functional connections whose strengths depend on the Euclidean distance between locations. In order to circumvent these problems, it has been suggested that zero-lag statistical dependencies should be removed altogether from FC analysis since signal leakage is instantaneous, resulting in measures such as imaginary coherence (Nolte et al., 2004) and phase lag index (Stam, Nolte, & Daffertshofer, 2007). Contrary to this, it has been pointed out that zero-lag statistical dependencies still carry meaningful information about ongoing activity (Pascual-Marqui et al., 2017; Tognoli & Kelso, 2009; Uhlhaas et al., 2009), and may therefore be particularly important for data recorded during resting state. Furthermore, approaches exist that orthogonalize the time series, removing common dependencies between sources (Brookes, Woolrich, & Barnes, 2012; Colclough, Brookes, Smith, & Woolrich, 2015; Hipp, Hawellek, Corbetta, Siegel, & Engel, 2012; Wens et al., 2015).
In this study, we propose an approach that incorporates additional information from SC into EEG functional connectivity on the source level. We first replicate the previous finding (Chu et al., 2015; Finger et al., 2016; Siems, Pape, Hipp, & Siegel, 2016; Wirsich et al., 2017) that SC partially shapes FC in EEG, beyond the impact of Euclidean distance induced by volume conduction. Subsequently, we use additional information in the SC to augment the EEG functional signal by applying a low-pass filter (or smoothing procedure) in the space spanned by the SC graph. The underlying motivation is that both SC and FC decay with increasing Euclidean distance. From an evolutionary standpoint, it makes sense for functionally related regions to be close together (Tomasi, Wang, & Volkow, 2013). As a result, it is impossible to disentangle the contributions of volume conduction and genuine FC mediated by SC to a statistical dependency measured between two brain regions. However, if one assumes that genuine FC is mediated by SC, and therefore genuine FC is higher between brain regions that are directly anatomically connected, increasing the impact of SC would also increase the contribution of genuine FC relative to FC generated by volume conduction. A similar approach has previously been shown to improve source estimation in EEG (Hammond, Scherrer, & Warfield, 2013).
We use an fMRI-FC acquired completely independently of the EEG dataset in order to determine whether our filtering procedure leads to improvements in terms of macroscopic network structure encoded in the FC. Numerous studies have shown that the macroscopic network structure is, on a coarse level, similar between fMRI and EEG (Britz, Van De Ville, & Michel, 2010; Coito, Michel, Vulliemoz, & Plomp, 2019; Liu, Ganzetti, Wenderoth, & Mantini, 2018; Musso, Brinkmeyer, Mobascher, Warbrick, & Winterer, 2010). These networks have been demonstrated to be relevant on several spatial and temporal scales of different recording techniques (Brookes et al., 2011; Kucyi et al., 2020; Liu, Farahibozorg, Porcaro, Wenderoth, & Mantini, 2017). We hypothesize that, if our filtering procedure indeed strengthens genuine FC mediated by SC, we should see that the network structure encoded in the EEG-FCs becomes more similar to that encountered in fMRI, a recording technique that is not impacted by volume conduction; we quantify this by testing whether the EEG-FCs computed from filtered time courses are more similar to fMRI-FC than the original, unfiltered signals, both overall and by explicitly analyzing the FC matrices’ community structure. Our results suggest that incorporating information from the SC by means of graph filtering leads to a large-scale network structure in EEG-FC that is more similar to known canonical resting-state networks, making our approach a possible alternative to other methods that aim at correcting for volume conduction, especially for the study of large-scale functional networks.
SC Provides Additional Predictive Power for EEG-FC
Our goal is to use the structural connectivity matrix to boost functional connectivity that is mediated by white matter anatomical connections in source-level EEG resting-state time series recorded from N = 18 subjects (see Figure 1 for an illustration of our approach).
One of our main assumptions is that genuine FC is in part mediated by SC (Chu et al., 2015; Finger et al., 2016; Wirsich et al., 2017). To test this assumption, we first show that SC can predict FC beyond the common dependence of FC and SC on Euclidean distance. We test this assumption by fitting a stepwise general linear model (GLM) in order to quantify how well the following measures predict EEG-FCs computed as envelope correlations from three typical EEG frequency bands (alpha: 8–13 Hz, beta: 13–30 Hz, gamma: 30–40 Hz), averaged over all subjects:
SC in the form of search information (Goñi et al. 2014), referred to as SCSI, a measure that is derived from fiber counts and that is nonzero for all connections, yielding a connectivity matrix that is dense just like the FC matrix (see Figure S1 in the Supporting Information); the intuitive interpretation of search information is that it measures how “hidden” the shortest path between two ROIs is. Note that search information and fiber count are roughly inversely proportional.
Euclidean distance (ED) between ROI centers.
Relative regional variance (i.e., the variance of each ROI time course, normalized such that the maximum variance in each subject equals 1) as an estimate of signal-to-noise ratio.
ROI size (number of voxels in the parcellation).
The third and fourth predictors are control variables for possible confounds. Note that in each case, we predict a dependent univariate variable—the EEG-FC—with an independent univariate variable, such that each pair of brain regions is a sample (i.e., we have (N × N − N)/2 samples). We analyze how well the independent variables can predict the FC in two different ways: On the one hand, we use each variable as the only predictor variable (plus intercept; blue bars in Figure 2; “single-variable model”). On the other, we test their predictive power when they are entered progressively into a GLM that includes first-order interaction terms (orange curves in Figure 2; “full model”). The latter case allows us to quantify the additional predictive power that each variable has, given all other predictors. This is important because the predictors are not independent of each other; most prominently, there is a three-way dependence between SC, Euclidean distance, and FC, such that both SC and FC decay with increasing Euclidean distance.
Figure 2A shows the results in terms of explained variance for the EEG-FCs and for comparison, for an fMRI-FC (average pairwise correlations over 88 subjects; see the Methods section for details; see Table S1 in the Supporting Information for detailed results of the GLM analysis). The correlation between the average EEG-FCs and the fMRI-FC is around 0.50 for all three bands (alpha, beta, gamma).
Figure 2A shows that ED is the strongest predictor in both EEG and fMRI. This is true for both the single-variable models (greatest explained variance as indicated by blue bars) and the full model (as indicated by the fact that they are the variables that are entered first into the model). However, the variance explained by ED is much higher in EEG than in fMRI, namely 0.55 in EEG (alpha band; other bands are similar, full list is in Table S1 in the Supporting Information) and 0.18 in fMRI. This is due to the effect of volume conduction, which introduces spurious correlations dependent on ED in the case of EEG-FC.
When using SCSI as the only predictor, we find that the dependency of FC on SCSI is very similar in both modalities (r2 of SCSI alone 0.14 in both modalities). In both cases, FC values are high between close-by pairs of brain regions (small ED; see Figure S2A in the Supporting Information). For fMRI, there are also highly correlated pairs that are separated by an intermediate distance, and close-by pairs that are barely or not at all correlated. In contrast, for EEG, all faraway pairs of ROIs have low correlations and all close-by pairs have high correlations. We checked for which connections the prediction of FC by SCSI was worst, that is, had the largest residuals (Figure S2B in the Supporting Information). We found that for fMRI, the largest errors occur on the secondary diagonal, replicating the well-known result that interhemispheric connections are underestimated in the SC. In EEG, this does not contribute as much to the unexplained variance, as the FC between homotopic regions is low compared with FC between close-by pairs of regions. In summary, while the variance explained by the SCSI is the same in both modalities, the structure of this dependency is different.
In both cases, SCSI explains an additional 3–4% of the variance (Table S1 in the Supporting Information) after regressing out Euclidean distance. For fMRI, some connections with intermediate distances remain SCSI as a predictor, again related to interhemispheric connections (Figure S2B, right panel). For EEG, the most severely underestimated FC values are related to small distances, indicating that neither Euclidean distance nor SCSI can by themselves account for some of the large EEG-FC values between nearby pairs of regions. Note that the actual contribution of SCSI is likely to be higher than 3–4%, as SC strength is itself dependent on Euclidean distance (two regions that are close together are more likely to be connected by white matter tracts; furthermore, short tracts are more easily traced by fiber tracking algorithms; Jones, 2010). Indeed, because of this mutual dependence, the actual contribution of SCSI cannot be estimated using this approach.
Furthermore, a significant positive interaction term between ED and SCSI contributes to the prediction in both fMRI and EEG. The correlation between these two variables (ED and SCSI) is 0.48, in line with previous findings (Wirsich et al., 2017). Since SC and SCSI are negatively correlated (see the Methods section), this translates to two interpretations: ED has less of an impact on the FC between ROI pairs that have a strong SC connection (high weight in the SC matrix); and the strength of the SC connection has less of an impact on the FC between ROI pairs that are far apart from each other (high ED).
A simple prediction from the hypothesis that FC is shaped by SC is that ROI pairs that are connected via white matter tracts should exhibit stronger FC than those that are not (Chu et al., 2015). In order to control for the common dependence of FC and SC on Euclidean distance, we compare average FC values over subsamples of pairs of ROIs that are matched in their Euclidean distance distribution. Figure 2B shows that even in those matched subsamples, there is indeed a significant difference between the mean FC values (Wilcoxon signed-rank test at alpha = 0.05, Bonferroni-corrected for multiple comparisons) between structurally connected and unconnected ROI pairs.
Graph Filtering Increases Resemblance Between EEG-FC and fMRI-FC
We filter the single epoch, source-level activity xi(t) of each ROI using the above formula and compute FC matrices from the filtered data (t) as before (envelope correlations in alpha, beta, and gamma frequency bands). We use four different graphs (Figure 3, from left to right):
The SC (number of fibers) itself, averaged across subjects according to Betzel, Griffa, Hagmann, and Mišić (2019). This graph has a connection density of 25%.
A graph derived from Euclidean distances, setting connection weights with exp(−k * ED), where the weight k just serves to scale the distribution such that the multiplication of the graph weights with the filter weights will result in effective weights in the same range as for the other SCs. This is a dense graph and will be referred to as “ED full.”
A graph derived from Euclidean distances, but exactly matching the SC in terms of existing and absent fibers. This means that the connections are the same as for SC 1, but the weights are set as in SC 2 instead of the fiber count. This graph will be referred to as “ED match.”
A graph derived from Euclidean distances, but preserving the density of SCs 1 and 3. This means keeping the connections that correspond to the smallest EDs up to a threshold, which leads to the same connection density as in SC 1 (and SC 3). We include this graph because otherwise, if matrices 1 or 3 outperform matrix 2, this could just be due to the difference in connection density. This graph will be referred to as “ED dens.”
After filtering, EEG-FCs are computed as for the unfiltered data. As expected, the filtered FCs become more similar to their respective SCSI (Pearson correlation between EEG-FCs and SCSI), and the difference in average correlation between structurally connected versus unconnected ROI pairs increases (see Figures S3 and S4 in the Supporting Information).
To validate our results, we compare our EEG-FCs with fMRI-FC (obtained by averaging over 88 subjects; see the Methods section for details) by computing the Pearson correlation between the two matrices for each subject. The goal is to assess whether the changes in FC induced by graph filtering result in a change in the network/community structure encoded in the FC that is in line with known functional networks (Britz et al., 2010; Coito et al., 2019; Liu et al., 2018; Musso et al., 2010). We obtain a comparison for each filter weight G and each of the four graphs described above and shown in Figure 3.
Figure 4A shows that correlations between the two matrices obtained from the two modalities increase as hypothesized (results are shown for beta band; alpha and gamma are similar [not shown]). The graph that reaches the highest maximum correlations between EEG-FC and fMRI-FC is the one in which Euclidean distance and SC are combined by masking the weights derived from Euclidean distances with the nonzero connections given by the SC (“ED match;” Wilcoxon signed-rank test comparing the correlation coefficients of each subject, p < 0.05 Bonferroni corrected). The mean correlation increases from 0.42 to 0.51 at a filter weight of G = 100, corresponding to a 23% increase (increase computed based on the Fisher z-transformed values as shown in Figures 4A and 4B: 0.46 and 0.56, respectively), while the increase when using the SC itself is only from 0.42 to 0.44 (Fisher z-transformed values: increase from 0.46 to 0.48). Figure 5 shows the original EEG-FC (beta band) and the EEG-FC derived from filtered data with G = 100.
In order to better interpret these results, we use a control SC in which connections are randomized while preserving the degree distribution and density (Figure S5 in the Supporting Information; see the Methods section for details). In this case, the fit between EEG-FC and fMRI-FC increases from 0.42 to 0.44 when using the SC itself, an increase that is almost the same that is original matrix, and that remains significant (Figure S5B in the Supporting Information; Wilcoxon signed-rank test, p < 0.05 Bonferroni corrected). The remaining three versions of the graph lead to statistically identical increases, that is, in this case, there is no advantage of using the SC-derived mask on the Euclidean distances (“ED match”) over using the full set of Euclidean distances (“ED full”) or the shortest Euclidean distances only (“ED dens”). Note that since randomizing the Euclidean distances is not readily possible (as the resulting geometry would need to be consistent), “ED dens” and “ED full” are identical for the randomized and the original SC.
Taken together, increases in fit due to the SC alone can mostly be attributed to the degree distribution (which is preserved in the random SC). Using a graph in which the real SC is used to mask Euclidean distance-derived weights leads to a significant advantage compared with purely Euclidean distance-derived graphs.
We further repeat the analysis using FC matrices computed from white Gaussian noise (WGN-FCs; see the Methods section) in order to test how far our results can be explained purely by linear dependencies imposed by the graphs used as filters. We find that the correlation between WGN-FCs that were filtered with the SC and the fMRI-FC reaches a maximum of 0.26 (Figure S6A in the Supporting Information), indicating that filtering white noise with the SC does not explain the association between fMRI-FC and EEG-FCs, where the correlation is 0.42 without any filtering (Figure 4A). The correlation between WGN-FCs and fMRI-FC reaches a value of r = 0.49 (z-transformed value: 0.53) when using only Euclidean distances (dense ED) with G = 500. This is comparable to the optimal fit of r = 0.51 (z-transformed value: 0.56) obtained with empirical EEG-FCs at G = 100 (ED match), but at G = 100, the empirical EEG-FCs clearly outperform the WGN-FCs (r = 0.35). We also checked whether the fit to fMRI can be explained by the fact that EEG-FCs become more similar to WGN-FCs as the filter weight is increased. At G = 500, the filtered EEG-FCs are very similar to the FCs obtained from filtered WGN (average r = 0.91 [z-transformed value: 1.5], Figure S6B in the Supporting Information). At G = 100, this correlation is r = 0.69 for ED match (z-transformed value: 0.85).
As a further comparison, we use the orthogonalization approach described in Colclough et al. (2015) to correct for leakage in the unfiltered data (Figure S7). We find that the correlation between fMRI-FCs and EEG-FCs decreases for each subject (Table S3). Furthermore, we repeat our analyses using coherence and imaginary part of coherence (Figures S8 and S9), the latter of which is assumed to remove zero-lag correlations resulting from volume conduction (Nolte et al., 2004). We find no advantage of these measures over correlations between power envelopes. Specifically, the correlation between fMRI-FCs and EEG-FCs computed using this measure is not higher than when using the power envelope correlations (Figure S8A).
High-Quality SCs Are Necessary to Achieve an Increase in Fit
We perform the same analysis with two additional SC matrices from different cohorts (see the Methods section for details). The first one is obtained from a small cohort with relatively lower quality than the “primary” SC used in the main analysis above, that is, diffusion tensor imaging (DTI) was used instead of diffusion spectrum imaging (DSI), as well as a smaller number of subjects (N = 20). The second one consists of the 45 subjects of the Human Connectome Project’s (HCP) “retest” dataset, data of very high quality. When using the lower quality data (Figure S10 in the Supporting Information), there is no additional benefit of using the SC matrix as a mask on the matrix of Euclidean distances (fit between EEG-FC and fMRI-FC is the same for “ED full” and “ED match”). However, when using the high-quality HCP SC matrices (Figure S11), ED match performs significantly better than the three other graphs, as is the case for the primary SC matrix. This also shows that our results do not depend on the fMRI data and the dMRI data stemming from the same subjects.
Figure S12A (in the Supporting Information) illustrates that the primary matrix used in this study, as well as the HCP matrix, possesses a higher density of interhemispheric connections than the DTI-derived matrix (primary: 12%, HCP: 20%, DTI: 9%, see Figure S12). This may be because interhemispheric fibers are found more consistently in the DSI-based datasets (primary but could also be attributed to variations in the tractography techniques (deterministic versus probabilistic, seeding from white matter versus seeding from gray matter/white matter interface). The absence of some interhemispheric connections leads to high errors in the resulting filtered EEG-FC for the DTI SC matrix (Figure S12B). This shows that our method relies in part on the improvement of interhemispheric FC, an effect that can only be achieved with high-quality diffusion data.
Graph Filtering Increases Resemblance Between EEG and fMRI Community Structure
In the following, we explore the effect of graph filtering on the EEG-FC structure. To this end, we use FCs averaged over all subjects. First, we consider seed correlations. In order to make correlations comparable, we resample average FCs such that FC values are normally distributed around mean 0 and with a standard deviation of 1. Figure 6 shows the normalized correlations between two seed regions and all other ROIs in the parcellation (i.e., one row/column of the EEG-FC) before and after filtering with the best SC identified above (“ED match,” G = 100; beta band). We choose these regions because they exhibit the largest overall change in connection weights with other regions (all changes in Figure S13 in the Supporting Information). In both cases, the correlations to the corresponding region on the other side of the brain are increased.
In general, the correlation structure of many regions changes considerably (Figure S13), but these changes are hard to interpret on a ROI-by-ROI basis. Therefore, to investigate this further, we extract the community structure of the average EEG-FCs before and after filtering by assessing the probability of any two regions to be assigned to the same community (Figure 7, top row and bottom left). We apply the same procedure to the average fMRI-FC (Figure 7, bottom right). As the filter weight (G) increases, more values in the “community matrices” tend towards 0 or 1, indicating that the variability in clustering outcomes decreases. Interestingly, also the community assignments derived from the fMRI-FC show some variability, especially for certain frontal and temporal regions. Figure 8A shows one example of community assignments. The main difference between EEG and fMRI is that EEG community structure is dominated by the lobe architecture, whereas in fMRI, we see the robust clustering of frontal with middle temporal and posterior cingulate cortices, reminiscent of the default mode network (Laird et al., 2009), as well as the symmetry across hemispheres between temporal regions.
We quantify the similarity between the community structures in EEG-FCs and fMRI-FCs by taking the rank correlation between each row/column of the “community matrices” (examples for λ = 1.1 in Figure 7). We compute this measure depending on two parameters: (a) the resolution parameter λ, which controls the spatial resolution of the Louvain algorithm and thus the number of communities; and (b) the filter weight G. Figure 9A shows the averaged (over ROIs) similarity in community structure. There is a region where the average rank correlation is ∼0.45, that is, for filter weights between 200 and 1,000 and λ = 1.075. At the same time, Figure 9B shows that with increasing λ, the number of communities also increases: The overall maximum in average rank correlation is 0.60 at λ = 1.3 and G = 800 (Figure S14), but at this point, we have 27 communities. Because of the coarseness of the parcellation used here, and according to the literature (Yeo et al., 2011), we seek to partition the cortex into as few communities as possible while also achieving a good agreement between the community structures of EEG-FCs and fMRI-FCs. Choosing λ = 1.1 (row indicated in Figures 9A and 9B), the number of communities is 5 and the agreement with the fMRI community structure is 0.45 (at G = 200, 300, or 400; see Figures 9C). The same fit can be achieved at λ = 1.125 and G = 300, but at this point we have 6.5 communities on average. Similarly, without filtering, the correlation is 0.42, but the number of communities is 6. Thus, λ= 1.1 and G = 200, 300, or 400 represents the optimal trade-off between number of communities and community agreement in comparison to other filter weights, including G = 0.
Since Figure 8A provides only an example partition, we plot on the cortex rows/columns of the community matrices in Figure 7 in order to analyze the community structure across all iterations of the clustering, that is, the probability of a specific ROI to be coassigned to the same community as each of the other ROIs. We call this the “community behavior” of this ROI. In Figure 8B, we show three ROIs that exhibit a switch in community membership pertaining to three major communities found by the Louvain algorithm: the frontal community, the somatosensory/motor network, and one of the two temporal communities; we do not show the occipital network as it is quite stable.
Figure 8B, left panel, shows how the “community behavior” of an example frontal ROI (right lateral orbitofrontal ROI) differs between unfiltered and filtered EEG-FCs. The most conspicuous change that is introduced by the filtering is the establishment of a coherent frontal network that includes orbitofrontal regions, as in fMRI. This can be observed in Figure 8A (blue network) as well as examining the changes in single ROIs displayed in Figure 9D.
Figure 8B, middle panel, shows an example from the somatosensory/motor network (right paracentral lobule). This ROI shows a marked improvement in its agreement with fMRI. Namely, before filtering, this ROI is grouped with frontal regions (dark blue network in Figure 8A). Afterwards, it becomes a member of the somatosensory/motor network (orange network in Figure 8A), as is the case in fMRI. However, in contrast to fMRI, a symmetrical network is not established. When using the SC graph for the smoothing procedure, this network is robustly expressed in a certain area of the parameter space (Figures S15 and S16 in the Supporting Information), however, the overall correspondence between EEG-FCs and fMRI-FCs and community structures is much lower at this point (average rank correlation: 0.22).
While the visual network is well established even without any filtering (Figure 8A, cyan network, and Figure 9D), temporal regions are mostly grouped because of their anatomical proximity. Figure 8B, right panel, shows how the community behavior of an example temporal region (right fusiform area) changes due to filtering. Despite there being a qualitative improvement in that the region switches to a temporal network and decouples from visual regions, there are also many spurious coassignments with other temporal regions that, in total, explain why this region shows no improvement due to filtering.
In this study, we show that additional information in the SC can be used to selectively increase the FC between brain regions that are connected via white matter fibers, leading to a large-scale functional network structure that is more in accordance with canonical resting-state networks (Liu et al., 2017; Yeo et al., 2011). We first show that the strength of FC between pairs of regions that are connected via SC is higher than between those pairs that are not, confirming the assumption that both volume conduction and SC contribute to Euclidean distance-dependent functional connectivity. Second, we find that using a graph that combines SC and Euclidean distances to smooth functional EEG signals in nearest neighbor graph space results in a higher agreement between fMRI-FC and EEG-FC structure.
SC Is Outperformed by Graphs (Partially) Derived from Euclidean Distance
The result that a combination of SC and Euclidean distance performs best was contrary to our initial expectation that SC (fiber counts) would give the largest improvement because of strengthening of FC between distant pairs of ROIs. SC is correlated with Euclidean distance with r = 0.48, and there exist many pairs of regions that are both nearby in terms of Euclidean distance and strongly connected according to the SC (Figure S2A in the Supporting Information). Thus, strong connections exist between these pairs in all four versions of the graph, and filtering based on any of the four graphs increases the strength of these connections (Figure S4). The increase in fit to the fMRI-FC common to all graphs indicates that despite the fact that these connections are already strong in the EEG-FCs, they are still underestimated compared with fMRI-FC. This is consistent with our rationale that strengthening these connections corresponds to boosting genuine FC mediated by white matter connections.
Furthermore, the GLM analysis showed that the third-strongest predictor for fMRI-FC was an interaction between SC and Euclidean distance, explaining why, beyond the commonalities across graphs based on Euclidean distance, the graph containing information from both SC and Euclidean distances outperforms all other graphs.
A trivial explanation for the increase in agreement between EEG-FC and fMRI-FC is that graph filtering removes pairwise nonlinear relationships that are only present in EEG but not in fMRI. Indeed, Messé, Rudrauf, Giron, and Marrelec (2015) showed that a generative model of fMRI-FC that takes into account only linear relationships between structurally connected pairs outperformed all other models that included nonlinearities. In order to investigate this possibility, we simulated white Gaussian noise (WGN) and applied the same filtering procedure, obtaining WGN-FC matrices. We found that at G = 100, where the best fit (r = 0.51) between EEG-FCs and fMRI-FCs is reached, the fit of WGN-FCs to fMRI-FCs is 0.35 (Figure S6A in the Supporting Information), despite the EEG-FCs being quite similar to filtered WGN-FCs (average maximum correlation: 0.69, Figure S6B). This suggests that making EEG-FCs more similar to linearly related WGN cannot fully account for the increase in fit between EEG-FC and fMRI-FC. However, at high G values (G > 500), the WGN-FCs did show a fit to the fMRI-FCs that was just as good as that obtained through our filtering procedure with the empirical EEG-FCs, namely around 0.5, which is also the correlation between fMRI-FC and Euclidean distance. At this point, the correlation between WGN-FCs and EEG-FCs reaches ∼0.9. This indicates that indeed, imposing an interesting graph structure on white Gaussian noise is able to account for about 25% of the variability in fMRI-FC. This result is in line with Messé et al. (2015). Importantly though, our filtering procedure identifies a range of G where only about half of this variance is explained by WGN, suggesting that some interesting nonlinearities are preserved.
It is worth mentioning that the results of both ED match and SC might be improved by optimizing the thresholding procedure. As there is currently no consensus on how thresholding of fiber count and recurrence of pairwise connections across subjects should be combined, how the differences between long and short fibers should be taken into account, and how thresholding procedures should differ depending on scanning protocols (e.g., DTI versus DSI), fiber tracking algorithms (e.g., probabilistic versus deterministic), and number of subjects, a systematic exploration of the effect of thresholding procedure is beyond the scope of this study. Nevertheless, the fitting procedure between EEG-FC and fMRI-FC described here could be employed as one measure to evaluate such thresholding procedures, as a higher fit could be taken to mean that the most relevant fibers were correctly preserved.
Comparison to Methods That Attenuate Volume Conduction
We used envelope-based correlations, which are known to be strongly influenced by volume conduction. We opted for this measure because it is widely used (Cabral et al., 2014; Hipp et al., 2012; O’Neill, Barratt, Hunt, Tewarie, & Brookes, 2015) and captures predominantly slow power modulations, which is closer to what the BOLD signal captures. Also, the graph filtering does not remove zero-lag correlations.
We repeated our analyses using coherence and imaginary part of coherence (Figures S8 and S9 in the Supporting Information), the latter of which is thought to remove zero-lag correlations and thus, volume conduction (Nolte et al., 2004). We found that the increase in fit to the fMRI-FC was much smaller. However, this was the case for both measures, and may therefore just reflect the fact that they are less suited for comparison with BOLD-FC. This does not mean that graph filtering does not work for coherence-based measures, but that comparing with fMRI-FC is not suitable in this case. Further research is necessary in order to clarify the effect of FC measure on graph filtering results. Qualitatively, the results were the same with all three measures.
We also used the method described in Colclough et al. (2015) to orthogonalize the EEG signals, and found that this resulted in a decrease in the correlations between EEG-FCs and fMRI-FCs for every subject, suggesting that orthogonalization removes genuine FC.
Overall, these results suggest that both orthogonalization and imaginary coherence remove zero-lag correlations relevant to large-scale network structure. This does not mean that these methods are not useful in detecting true connectivity (Nolte et al., 2004). Some recent studies using such measures have shown network structure partly concordant with fMRI resting-state networks, and have added some directionality in network analysis (Coito et al., 2016, Coito et al., 2019; Silfverhuth, Hintsala, Kortelainen, & Seppänen, 2012).
Euclidean Distance and Fiber Count Differentially Affect FC in Different Communities
Our community analysis, using Louvain clustering, revealed that EEG functional networks are differentially affected by our filtering procedure (using “ED match”). For frontal regions, robust improvements are observed, yielding a network that resembles that found in fMRI, apart from missing functional connections that would constitute the hallmarks of the default mode network, that is, long-range connections between frontal regions and the middle temporal, inferior parietal, and posterior cingulate cortices. However, note that with this coarse anatomical—not functional—parcellation, even the fMRI community structure, does not clearly resolve the default mode network, which is mixed with the frontoparietal network. This indicates that for this network, “ED match” is a good choice.
For temporal regions, fMRI shows a distinct structure that is not reproduced by EEG either with or without filtering. The main difference is that there is no FC across hemispheres between temporal regions. This shows a limitation of our approach (see below for more discussion on limitations), because even in the SC, there are very few, if any, white matter fiber tracts between the temporal lobes. This is because these fibers are very long and pass through the corpus callosum, making them hard to track. Beyond that, in fMRI, superior, middle, and inferior temporal gyri belong to different networks (somatosensory/motor/auditory, default mode/frontoparietal, limbic/visual, respectively—the resolution of this parcellation is too coarse to resolve these systems properly), while in EEG, the anatomical architecture of the lobes determines the partition into communities. This is a shortcoming that could potentially be improved by using a more EEG-appropriate parcellation.
Finally, for parietal regions typically belonging to a prominent somatosensory/motor network, improvements are achieved using “ED dens;” however, the typical symmetric network including pre- and postcentral gyri is not established. This is because the pre- and postcentral gyri are elongated, and therefore the Euclidean distances between ROI centers—which were used to establish the weights in the “ED match” graph—are quite high. Therefore, for this network, SC is the better choice, even though overall SC is outperformed by all ED-based graphs.
These observations are in line with recent findings that show that the alignment between SC and FC, that is, the degree to which FC is shaped by SC, differs across regions (Preti & Van De Ville, 2019), with sensory regions—that is, the visual and somatosensory/motor cortices—being more strongly aligned with the SC than higher cognitive areas. It may also correspond to a cortical gradient showing different levels of local recurrent connections across functional networks (Wang et al., 2019).
Previous studies have shown consistent functional connectivity between certain homotopic regions (Hipp et al., 2012; Mehrkanoon, Breakspear, Britz, & Boonstra, 2014), while here and elsewhere (Cabral et al., 2014) these connections are shown to be underestimated in M/EEG-FC. On the one hand, these findings might depend on the exact methodology (e.g., which source-reconstruction algorithm is used, whether signals were orthogonalized or not). On the other hand, our finding that frontal/occipital networks are quite symmetric across hemispheres even before filtering while temporal and parietal networks are not suggests that FC might be robust between some regions and largely absent between others.
We have already mentioned two limitations of our methodology. First, the SC has obvious shortcomings like the absence of many interhemispheric fibers and the underestimation of long fibers, which are hard to track (Jeurissen, Descoteaux, Mori, & Leemans, 2019; Jones, 2010). Second, we used envelope-based correlations, which are known to be strongly influenced by volume conduction. Although we compared with coherence and imaginary part of coherence, a closer investigation of how graph filtering impacts different measures is warranted.
Additionally, the standard FreeSurfer parcellation is probably not optimal for EEG. The ROIs of this parcellation are mostly anatomically defined, not taking into account the nature of the EEG signal: Regions are highly unequal in size, resulting in a wide range of numbers of dipoles being averaged to obtain the ROI time courses. Furthermore, ROIs are in many cases elongated, while for EEG, more spherical regions (as far as this would be anatomically/functionally plausible) would be preferable. Finally, the appropriate number of ROIs is a matter of debate, as a simple correspondence between the number of ROIs and the number electrodes is not applicable (Farahibozorg, Henson, & Hauk, 2018). This is also in line with our finding that in the GLM, the relative regional variance (RRV) is the second-strongest predictor of EEG-FC in terms of added explained variance, indicating that noise is unequally distributed across ROIs. This could be because signals from deep sources are harder to pick up than those of superficial sources (Whittingstall, Stroink, Gates, Connolly, & Finley, 2003).
On the conceptual level, it is unclear how much EEG-FC should resemble fMRI-FC, since BOLD and the EEG signal are related in a way that is not straightforward. At the same time, recent studies show that resting-state networks are quite similar across these modalities and therefore, on this level of resolution and detail, we should expect a good agreement between the FC matrices (Coito et al., 2019; Liu et al., 2018). Still, validation should include a biophysical model that simulates both genuine FC based on SC as well as volume conduction.
Conclusions and Future Work
Taken together, we add to the thus far sparse knowledge on how SC and FC are related in EEG source space. Developing these methods is crucial for taking full advantage of the immense richness of the EEG signal in the temporal and frequency domain, and combining EEG with other modalities like MEG and fMRI. We have limited our analysis to the grand-average FC, but our method could be used to improve signal-to-noise ratio on the single trial level, potentially easing statistical analysis of task EEG in source space. One important conclusion from our results is that EEG-specific parcellation schemes are necessary to guarantee that we take full advantage of the richness of the EEG signal. Furthermore, our results confirm that it is feasible and sensible to use dynamical models that assume functional activity to spread through white matter fibers in EEG (Bhattacharya et al., 2011; de Haan et al., 2012; Finger et al., 2016; Pons et al., 2010; Ponten et al., 2010; van Dellen et al., 2013). On the data-analysis side, this study provides a justification and an avenue to applying more sophisticated methods like graph signal processing (Shuman, Narang, Frossard, Ortega, & Vandergheynst, 2012).
EEG Data and Source Projection
Data were recorded from 21 healthy controls as part of an epilepsy study at the EEG and Epilepsy Unit, University Hospitals of Geneva. The local ethics committee approved this study. Three subjects were excluded because of too many movement artifacts, leaving 18 for analysis. Since subjects were age-matched to patients (not analyzed here), 6 subjects aged less than 18 years were included (age range: 8 to 54 years, median: 29.5). Since we could not find any qualitative differences when excluding these subjects, we proceeded with using all 18 available datasets.
Resting-state EEG was collected with the Geodesic Sensor Net with 256 electrodes (Electrical Geodesic, Inc., Eugene, USA) during resting state. Electrodes on cheeks and neck were excluded, leaving 204 electrodes for analysis. Data were downsampled to 1 kHz and artifacts were removed by Infomax-based independent component analysis prior to source projection. Remaining artifacts were marked manually and visually, and markers were later used to extract artifact-free intervals of varying length and number per subject (Table S3 in the Supporting Information). Inverse solutions were computed using LAURA with LSMAC as implemented in CARTOOL (Brunet, Murray, & Michel, 2011), employing individual head models that were extracted from T1-weighted images (acquired as magnetization-prepared rapid gradient echo [MPRAGE] volumes with a Siemens TrioTim 3T MRI scanner and a tfl3d1ns pulse sequence with flip angle = 9°; echo time = 2.66 ms, repetition time = 1.51 s, inversion time = 0.9, voxel size = 1 × 1 ×1 mm3 head first supine) obtained from the same subjects in order to create the forward model. Segmentation and ROI extraction (i.e., parcellation) was performed using Connectome Mapper 3 (Tourbier et al., 2020). Gray and white matter were segmented from the MPRAGE volume using FreeSurfer with the Lausanne 2008 multiscale parcellation (Hagmann et al., 2008), whose first scale corresponds to the Desikan atlas (Desikan et al., 2006).
Data were source projected to ∼5,000 dipole locations equally spaced on a three-dimensional grid, where the gray matter volume extracted from the same images served as a constraint for the dipole locations. In order to project the three-dimensional time courses of the solution points to one-dimensional ROI time courses for further analysis, the main direction of variance was extracted using singular value decomposition (Rubega et al., 2019): All solution points were concatenated and their time courses were projected onto the first principal component, preserving most of the variance. This was done for each artifact-free interval (Table S3 in the Supporting Information). Note that all analysis steps were done on the individual level. Since both functional (see below) and structural connectivity were computed between ROIs, averaging of the corresponding FC and SC matrices is possible without ever coregistering images to a common space.
We used three separate structural connectivity datasets in this study, one primary and two additional datasets to check for replicability. See Figure S12 in the Supporting Information for a comparison between the average SC matrices resulting from these datasets.
Primary structural connectivity matrices and fMRI functional connectivity matrices: 88 healthy control subjects (mean age 29.7 years, minimum 18.5 years, maximum 59.2 years; 34 females) were scanned in a 3-Tesla MRI scanner (Trio, Siemens Medical, Germany) using a 32-channel head coil. Informed written consent in accordance with institutional guidelines (protocol approved by the Ethics Committee of Clinical Research of the Faculty of Biology and Medicine, University of Lausanne, Switzerland, #82/14, #382/11, #26.4.2005) was obtained for all subjects. A large subset (70 out of 88 subjects) of the structural and functional connectivity matrices obtained from these data are available on Zenodo (Griffa, Alemán-Gómez, & Hagmann, 2019), where the processing pipelines are described in detail. Briefly, for diffusion imaging, a DSI sequence (128 diffusion-weighted volumes and a single b0 volume, maximum b-value 8,000 s/mm2, 2.2 × 2.2 × 3.0 mm voxel size) was applied. For resting-state fMRI, a gradient echo EPI sequence sensitive to BOLD contrast (3.3-mm in-plane resolution and slice thickness with a 0.3-mm gap, TR 1,920 ms, resulting in 280 images per participant) was applied. A magnetization-prepared rapid acquisition gradient echo (MPRAGE) sequence sensitive to white/gray matter contrast (1-mm in-plane resolution, 1.2-mm slice thickness) was also acquired. ROI extraction (i.e., parcellation) was performed using the Connectome Mapper (Daducci et al., 2012). Gray and white matter were segmented from the MPRAGE volume using FreeSurfer with the Lausanne 2008 multiscale parcellation (Hagmann et al., 2008), whose first scale corresponds to the Desikan atlas (Desikan et al., 2006). DSI data were reconstructed following the protocol described in Wedeen, Hagmann, Tseng, Reese, & Weisskoff (2005).
Structural connectivity matrices were estimated for individual participants using deterministic streamline tractography on reconstructed DSI data, initiating 32 streamline propagations per diffusion direction, per white matter voxel (Wedeen et al., 2008).
FMRI volumes were corrected for physiological variables, including regression of white matter, cerebrospinal fluid, as well as motion (three translations and three rotations, estimated by rigid body coregistration). Time courses of voxels falling into each ROI were average and FC was computed as correlations between these ROI time courses.
Control structural connectivity matrices, small DTI cohort: 20 healthy control subjects (17 females, mean age: 23, age range: 20–29 years) were scanned in a 3-Tesla MRI scanner (DISCOVERY MR750, GE Healthcare, USA) using a 32-channel head coil. For diffusion imaging, a DTI sequence (30 diffusion-weighted directions with b-value 1,000 s/mm2 and five b0 volumes, with 1 × 1 × 2.2 mm3 voxel size, TE/TR = 87/8,000 ms) was applied. A T1-weighted (T1w) volume (1-mm isotropic resolution) was also acquired, using an inversion-recovery-prepared fast spoiled gradient recalled brain volume (IR-FSPGR-BRAVO) sequence, a fast SPGR sequence with parameters tuned to optimize brain tissue contrast. ROI extraction (i.e., parcellation), diffusion signal analysis, and construction of SCs were performed using the Connectome Mapper 3 (Tourbier et al., 2020). Gray and white matter were segmented from the T1w volume using FreeSurfer and the Lausanne 2008 multiscale parcellation scheme (Hagmann et al., 2008). DTI data were corrected for motion and distortions using FSL mcflirt (Jenkinson, Bannister, Brady, & Smith, 2002) and eddy_correct (Andersson & Sotiropoulos, 2016), from which fiber orientation distribution functions (fODFs) were estimated via constrained spherical deconvolution (order 4). Then, by seeding from the white matter voxels and by the means of fODF-driven deterministic tractography (MRtrix3 SD_STREAM, Tournier et al., 2019, a total of 1M streamlines were reconstructed. T1w and b0 mean volumes were nonlinearly coregistered using ANTs (Avants, Epstein, Grossman, & Gee, 2008)) symmetric normalization in order to project the extracted ROIs to the DTI data space. Finally, by identification of the ROIs connected by fibers (Hagmann et al., 2008), a graph is constructed and the structural connectivity matrices are estimated for each individual.
Control structural connectivity matrices, Human Connectome Project Retest cohort (Feinberg et al., 2010; Moeller et al., 2010; Setsompop et al., 2012; Van Essen et al., 2012): The HCP Retest cohort consists of a subset of 45 HCP subjects recruited by the HCP to undergo the HCP 3T scanning pipeline a second time. The HCP uses a customized Siemens 3T “Skyra Connectome” scanner with a 32-channel head coil, which is located at Washington University in St. Louis, USA, and which provides an increased gradient strength compared with standard scanners. This allows the acquisition of multishell dMRI data (voxel size: 1.25 × 1.25 ×1.25mm3). Ninety diffusion weighting directions and six b0 volumes were acquired for three different shells (with b = 1,000, 2,000, and 3,000 s/mm2, voxel size: 1.25 × 1.25 × 1.25 mm3; Feinberg et al., 2010). An MPRAGE image is also acquired sensitive to the white/gray matter contrast (0.7-mm isotropic resolution) and used for ROI extraction. In this work we used in particular the publicly available T1w- and dMRI-derived data preprocessed by the “HCP minimal preprocessing pipelines” described in Glasser et al. (2013). This consists of (a) the T1w images, (b) the corresponding processed FreeSurfer outputs, and (c) the fully corrected dMRI images. ROI extraction (i.e., parcellation), diffusion signal reconstruction and tractography, and construction of SCs were also performed using the Connectome Mapper 3 (Tourbier et al., 2020). Gray and white matter were segmented using the existing precomputed FreeSurfer outputs and the Lausanne 2008 multiscale parcellation scheme. Fiber orientation distribution functions were estimated from the existing preprocessed dMRI volumes via constrained spherical deconvolution (order 10). Then, by the means of anatomically constrained and fODF-driven probabilistic tractography (MRtrix3 ACT iFOD2, ACT; Smith, Tournier, Calamante, & Connelly, 2012) with seeding from the white matter interface, a total of around 200,000 streamlines were reconstructed. T1w and b0 mean volumes are nonlinearly coregistered using ANTs symmetric normalization, and the extracted ROIs are projected to the dMRI data space. Finally, by identification of the ROIs connected by fibers, a graph is constructed and the structural connectivity matrices are estimated for each individual.
Functional Connectivity Measures for EEG
For our main analysis, we used power envelope correlations to measure functional connectivity in alpha (8–13 Hz), beta (13–30 Hz), and gamma (30–40 Hz) bands. All data analysis was performed in Matlab 2017a (MathWorks, Natick, USA). We extracted artifact-free intervals from the resting-state time courses by using the artifact markers mentioned above, admitting only intervals of at least 19-s duration in order to guarantee a reliable estimation of slow envelope modulations (see below). Prior to extracting the FCs, for each artifact-free interval, the data were downsampled to 250Hz in order to avoid numerical errors that can be induced if the band of interest is much lower than the sampling rate.
Envelope correlations were computed as in Cabral et al. (2014): After zero-phase band-pass filtering (eegfilt from EEGLAB software package) the EEG single trial time series in the respective bands, envelopes were extracted using the Hilbert transform. Envelopes were again low-pass filtered at 0.5 Hz, capturing ultraslow modulations in the respective bands. This measure was chosen to be as closely related as possible to fMRI-FC (Pearson correlation between BOLD time courses).
As a comparison (see the Discussion section), we computed the EEG-/fMRI-FC correlations also using FCs obtained with coherence and imaginary part of coherence (icoherence; Nolte et al., 2004). This part of the analysis was performed using functions implemented in the Brainstorm toolbox (Tadel, Baillet, Mosher, Pantazis, & Leahy, 2011), which is documented and freely available for download online under the GNU general public license (http://neuroimage.usc.edu/brainstorm). Brainstorm estimates (i)coherence in a minimum number of five windows that overlap by 50%. At a sampling rate of 250 Hz, this results in a minimum required signal length of ∼3 s. Thus, artifact-free intervals were segmented into epochs of ∼3 s. Since we found that these measures were more susceptible to noise introduced by remaining artifacts than envelope correlations, we further rejected epochs that, at any point, deviated from the mean by more than 6 standard deviations. See Table S3 in the Supporting Information for the number of epochs that was used for each subject.
Average Structural Connectivity Matrices
All data analysis was performed in Matlab 2017a (MathWorks, Natick, USA). We used the method introduced in Betzel et al. (2019) to obtain unbiased group-consensus SCs (average number of fibers) for all three datasets used in this study (primary, HCP, and DTI; see above). The reason for using a group average rather than individual SC matrices is that fiber tracking algorithms are not sufficiently reliable on an individual level, and information from the entire group is necessary to identify the most likely true-positive connections.
In brief, this method takes into account the fact that interhemispheric connections are less reliable than intrahemispheric ones. Using a single threshold on the average SC strength (i.e., setting all connections that have less than a certain average number of fibers to 0) results in an underestimated interhemispheric connection density compared with single subjects. The method used here (Betzel et al., 2019) preserves both intra- and interhemispheric connection density found in single subjects by applying separate thresholds. Additionally, we require a recurrence of at least 30%. The resulting connection density in our primary SC is 25% (39% within and 12% across hemispheres).
Applying the distance-dependent threshold (Betzel et al., 2019) and a recurrence threshold of 0.3 to the additional datasets, DTI and HCP as described above, resulted in different connection densities (DTI: 22% overall, 36% within and 8% across hemispheres; HCP: 50% overall, 67% within and 34% across hemispheres). We adjusted the parameters of the distance-dependent thresholding procedure such that the resulting average SC exhibited a density that, for the DTI dataset, was above the density of the single subjects (18% higher), and for the HCP dataset such that it was below the single subject (41% lower). In order to still be able to take advantage of the superior quality of the HCP data compared with the DTI data, we chose to match the intrahemispheric connection density, allowing the interhemispheric, and thus, the overall density, to vary. This resulted, for DTI, in an overall density of 24% (39% within and 9% across hemispheres), and for HCP, in an overall density of 30% (39% within and 20% across hemispheres).
Lastly, the randomized SC used in one of our control analyses was obtained using the Brain Connectivity Toolbox (Rubinov & Sporns, 2010) function randmio_und_connected() with 100 iterations, which we used to randomly rewire the primary SC 100 times, thereby preserving the degree sequence.
General Linear Model
We use Matlab’s function stepwiseglm() to compute coefficients and p-values for a general linear model (GLM) that computes FC from four predictors:
search information derived from SC, as detailed above;
relative regional variance (RRV); and
RRV serves as a proxy for signal-to-noise ratio. First, we compute for each ROI and each subject the average (over intervals) variance of this ROI’s time courses. The variances are then scaled such that the maximum (over ROIs) variance is 1 and averaged over subjects.
Matlab’s stepwiseglm() finds the order of predictor variables in terms of their deviances, that is, twice the difference between the log-likelihood of that model and the full model (using all possible predictors, i.e., four main effects and six interaction terms). A variable is added into the model if the difference between deviances obtained by adding it is significant (χ2 test). Removal of a term is also possible; this can occur if predictors are linearly dependent. Any nonsignificant predictors are not included. Interactions are included only if the main effects are significant. For comparison, we fit single-variable GLMs that use only the intercept and one predictor variable at a time (see Figure 2A).
Smoothing in Graph Space
Statistical Analysis for Comparing EEG-FC and fMRI-FC Matrices
We compute individual FC matrices after smoothing in graph space of each subject’s EEG node time courses. Each subject’s FC matrix, for each value of G, was correlated to the group average fMRI-FC matrix. Since the fMRI-FC matrix was computed from a rather large sample (88 subjects), we assume that it represents a “canonical” functional network structure. In order to assess whether the filtering procedure increases each subject’s EEG-FC similarity to this canonical network structure, we compared the best fit resulting from each of the four versions of the graph with the baseline as well as among each other, using a Wilcoxon signed-rank test. This is a paired test, comparing the differences of each subject’s individual correlations against 0. Since there are 10 comparisons (each of the four graph versions against baseline, and seven pairwise comparisons between graph versions), we just show a summary of the results as boxplots; however, note that we did not compare the means or medians, but the individual differences.
Functional Connectivity Matrices Derived From White Gaussian Noise
In order to explore the effect that smoothing in graph space is likely to remove interesting nonlinearities in EEG-FC, we created white Gaussian noise (WGN) time series using Matlab’s wgn() function. We preserved the power (variance) of the original signals for each artifact-free interval separately and computed FC in exactly the same way as for the EEG data. In addition to assessing the correlation between these WGN-FCs and fMRI-FCs, we quantified how much filtered EEG-FCs resemble WGN-FCs by computing for each filter weight the correlation between the EEG-FCs filtered with this weight and all filtered WGN-FCs, as a match between the weights cannot be assumed. In Figure S6B (see the Supporting Information), the maximum correlations to any filtered WGN-FC are shown.
Analysis of Community/Modular Structure of FC Matrices
We used the Louvain community detection algorithm implemented in the Brain Connectivity toolbox (Rubinov & Sporns, 2010) to evaluate the community structure present in the FC matrices. This algorithm takes the FC (adjacency) matrix and assigns each ROI to a community. The function takes one parameter, γ, which controls the spatial resolution and thus, indirectly, the number of communities that are detected, with higher values of γ leading to more and smaller communities. We varied γ in a range of 0.9 ≤ γ ≤ 1.3, resulting in a minimum of 2 and a maximum of 35 communities (Figure S14 in the Supporting Information).
Since the community structure depends to some degree on the initial conditions (randomly assigned module memberships of ROIs), we repeated the procedure 200 times. Instead of opting for a “hard assignment” approach, we took into account the uncertainty of the cluster structures. For each round of clustering, we determined for each pair of ROIs whether they were assigned to the same community. This resulted in a “community matrix” that can be interpreted as indicating the probability of a pair of ROIs to belong to the same community. Thus, each row/column of one of these matrices describes the “community profile” of a given ROI. In order to compare the community structures of EEG-FCs vs fMRI-FCs, we compute the rank correlations between the community profiles (row/column of community matrix) of each ROI.
Supporting information for this article is available at https://doi.org/10.1162/netn_a_00147.
Katharina Glomb: Conceptualization; Formal analysis; Investigation; Methodology; Software; Validation; Visualization; Writing - Original Draft; Writing - Review & Editing. Emeline Mullier: Conceptualization; Data curation; Investigation; Methodology; Software; Writing - Review & Editing. Margherita Carboni: Data curation; Formal analysis; Methodology; Software; Writing - Review & Editing. Maria Rubega: Data curation; Methodology; Software; Writing - Review & Editing. Giannarita Iannotti: Data curation; Writing - Review & Editing. Sebastien Tourbier: Data curation; Writing - Review & Editing. Martin Seeber: Data curation; Writing - Review & Editing. Serge Vulliemoz: Conceptualization; Methodology; Writing - Review & Editing. Patric Hagmann: Conceptualization; Funding acquisition; Methodology; Project administration; Resources; Supervision; Writing - Original Draft; Writing - Review & Editing.
Patric Hagmann, Katharina Glomb, Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (http://dx.doi.org/10.13039/501100001711), Award ID: 170873. Maria Rubega, Ministero degli Affari Esteri e della Cooperazione Internazionale, Award ID: PGR06121. Serge Vulliemoz, Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (http://dx.doi.org/10.13039/501100001711), Award ID: 169198.
- EEG source imaging:
The projection of EEG signals recorded on the scalp into the gray matter by means of mathematical models.
- Functional connectivity:
Statistical relationship (for example, correlation or coherence) between time series recorded from pairs of brain regions (parcels).
- Structural connectivity:
Using specific imaging techniques, pathways of axon bundles connecting brain regions (parcels) over large distances are traced and summarized in SC matrices.
- Graph filtering/smoothing:
A weighted average of a network node’s signal and the signals of its neighbors as defined by the graph.
- Envelope correlations:
A type of functional connectivity that considers correlations of slow fluctuations only.
A partition of the brain into functionally and anatomically more or less homogeneous brain regions, or parcels.
- Network communities:
A partition of a network into sets of nodes (here: brain regions) that are more connected (as quantified by functional/structural connectivity) to each other than to other parts of the network.
Competing Interests: The authors have declared that no competing interests exist.
Handling Editor: Richard Betzel