Abstract
The dynamic repertoire of functional brain networks is constrained by the underlying topology of structural connections. Despite this intrinsic relationship between structural connectivity (SC) and functional connectivity (FC), integrative and multimodal approaches to combine the two remain limited. Here, we propose a new adaptive filter for estimating dynamic and directed FC using structural connectivity information as priors. We tested the filter in rat epicranial recordings and human event-related EEG data, using SC priors from a meta-analysis of tracer studies and diffusion tensor imaging metrics, respectively. We show that, particularly under conditions of low signal-to-noise ratio, SC priors can help to refine estimates of directed FC, promoting sparse functional networks that combine information from structure and function. In addition, the proposed filter provides intrinsic protection against SC-related false negatives, as well as robustness against false positives, representing a valuable new tool for multimodal imaging in the context of dynamic and directed FC analysis.
INTRODUCTION
The white matter architecture of the human brain constitutes the structural backbone for neuronal communication. A fixed network of axonal pathways wires an extremely rich repertoire of brain functions, from short-range interactions to large-scale dynamics that support perception, cognition, and action (Petersen & Sporns, 2015). As in all biological systems, properties of the structure constrain the possible functions. Organizational principles of structural brain networks, such as small-world and modular architectures, determine the topological space for functional interactions at the meso- and macroscale (Hagmann et al., 2008; Sporns, 2010). At the microscale, the absence of a synaptic connection between two neurons makes a direct functional coupling biologically impossible. Despite the inherent link between structural connectivity (SC) and functional connectivity (FC), only a few existing studies have tried to combine the two modalities, particularly in the domain of directed and dynamic functional connectivity (Lei et al., 2015; Suárez et al., 2020).
The relationship between structural wiring and functional coupling is at the core of several statistical and biophysical models of brain networks (Honey et al., 2010). These models advocate a substantial overlap between SC and FC, at both the mesoscopic and the macroscopic scales. Empirical and modeling studies on resting-state brain networks provide converging support, showing that the weights of structural and functional networks, as well as their topological features, tend to be correlated, and the strength of between-regions SC is typically a good predictor of their FC (Deco et al., 2013; Honey et al., 2009; Mišić et al., 2016; Skudlarski et al., 2008). Given the established overlap between structure and function, measures of FC may be meaningfully improved by taking SC into account, as suggested by a few proposed methods. In the framework of Bayesian modeling, for instance, structural graphs have been incorporated as priors for generative models of FC (Sokolov et al., 2019). A structural graph is typically obtained from in vivo diffusion-weighted imaging (DWI) data that quantify the anisotropy in the diffusion of water molecules along white matter tracts (Hagmann et al., 2008). The connectivity graph is either a binary or a weighted undirected adjacency matrix that provides information about the presence and strength of a physical link between distinct brain regions. It has been shown how adding SC graphs as priors for effective connectivity substantially improves model evidence (Sokolov et al., 2019). Similarly, constraining FC for only present SC links and anatomically determined time lags may reduce false positives and improve the spatial resolution of electroencephalography (EEG) source imaging (Filatova et al., 2018; Takeda et al., 2019).
Whereas previous work has focused mainly on combining SC and FC for the analysis of functional magnetic resonance imaging data (fMRI), similar integrative approaches are missing for the emerging field of time-varying directed FC analysis (Eichenbaum et al., 2021). Time-varying FC has been used to characterize the dynamics of directed neuronal interactions that evolve at the millisecond scale, exploiting high-temporal-resolution recordings, such as local field potentials and EEG source imaging data (Milde et al., 2010; Pascucci et al., 2018; Plomp et al., 2014).
Recently, we introduced a variant of the classic Kalman filter, the self-tuning optimized Kalman (STOK) filter (Pascucci et al., 2020), for modeling rapid changes in large-scale functional networks during evoked brain activity. Here, we present an extension of this algorithm that incorporates prior information on the structural connectivity: the structurally informed STOK (si-STOK). The algorithm provides a straightforward tool to combine SC (e.g., diffusion tensor imaging [DTI]-derived metrics) with dynamic FC. In the proposed approach, SC priors act as regularization terms in the estimation of “effects,” within the Granger causality framework (Granger, 1969). Hence, the method is different from generative and predictive models that use SC as priors on the generation of neuronal dynamics and interregional coupling (Stephan et al., 2009). The underlying assumption of our method is that statistical influences among brain regions must follow the direct and directed nature of brain interactions, which occur between nodes of a network that are structurally linked. Hence, SC priors provide information about the fixed structure of directed links in which dynamic FC is likely to occur, without forcing FC, at any time point, to be necessarily identical to SC (see Methods).
We tested the algorithm in benchmark data and evaluated the effect of different SC matrices on the estimated FC. We demonstrated the advantages of combining SC with FC in terms of noise resilience and consistency of the estimates. We then compared the two algorithms in event-related large-scale functional brain networks during face processing. Our results showed that incorporating SC in dynamic FC promotes sparser and physiologically plausible topologies of functional networks, aiding the identification of the main network drivers and dynamics. MATLAB and Python code for si-STOK are available on GitHub (Pascucci & Rubega, 2019, https://github.com/PscDavid/dynet_toolbox; Rué-Queralt, 2019, https://github.com/joanrue/pydynet).
RESULTS
Somatosensory Evoked Potentials in Rats
To incorporate structural priors in dynamic functional connectivity, we developed a variant of the self-tuning optimized Kalman (STOK) filter (Pascucci et al., 2020). The STOK is an adaptive filter that derives time-varying multivariate autoregressive (tv-MVAR) coefficients using a simple least squares regression of present on past signals. We exploited this core feature of the filter to incorporate SC as shrinking priors of a least squares solution (see Methods, Equation 7). SC matrices were used to calibrate the variance of a prior expectation of zero FC between each pair of nodes (Sokolov et al., 2019; Figure 1). Strong SC values correspond to large prior variance, allowing FC to deviate from zero, whereas weak SC values reduce the prior variance and shrink FC toward zero. Hence, the filter’s estimates combine the strength of SC with the FC supported by the data (see Methods and Figure 1).
We tested the proposed algorithm, termed structurally informed STOK (si-STOK), on a benchmark dataset of epicranial EEG recordings in rats, from a whisker stimulation protocol (Plomp et al., 2014; Quairiaux et al., 2011). After whisker stimulation, action potentials originate and propagate rapidly (e.g., within 10–25 ms) from the contralateral primary sensory cortex, following the underlying structural connectivity (Plomp et al., 2014) (see Figure 2A). This pattern was accurately recovered by the STOK filter, which detected an overall larger magnitude of outgoing directed influences (MDI; see Methods) from the contralateral sensory cortex (e4), at early poststimulus latencies (13 ms; see Figure 2A and Pagnotta et al., 2018; Pagnotta & Plomp, 2018; Plomp et al., 2014). We used a directed, weighted matrix of SC that we derived from a meta-analysis of reported structural connections (Bota et al., 2015; Swanson et al., 2017) (see Methods). Compared with the regular STOK filter, the inclusion of SC priors provided qualitatively similar results, with clearer dynamics and visible but minor changes (Figure 2A). The similarity between the results of the two algorithms was a consequence of the use of a dense SC matrix, with connection weights that did not deviate drastically from the estimated FC. When evaluating the magnitude of outgoing influences from e4 to all the other nodes, indeed, the results were highly comparable (Figure 2B). However, incorporating a sparser SC matrix, with only 25% of the strongest SC connections, led to evident changes. When one of the expected FC connections (from e4 to e2) was absent in SC, the resulting estimate decreased considerably (Figure 2B). Nevertheless, FC from e4 to e2 was still larger compared with two other SC-absent connections (from e4 to e1 and from e4 to e8) for which weak or no FC was supported by the data. Conversely, for connections with weak FC, strong SC did not drastically increase FC. This demonstrated that in the present modeling framework the inclusion of structural priors has a low risk of producing false negatives (the downscaling of FC for absent SC depends also on the strength of FC), while it is also robust against the risk of introducing false positives driven by strong SC in the absence of FC.
To better appreciate the advantages of combining SC and FC, we compared the performance of the two algorithms under noise perturbations. We evaluated two criteria (see Methods): (a) the consistency of the estimated network at the e4 peak latency; and (b) the ability to detect e4 as the main driver compared with the average network activity. The two criteria were tested by varying the ratio of noise to signal. As evident in Figure 2C, the si-STOK outperformed the regular STOK for both criteria at all the noise levels. This highlighted an additional important feature of the new filter: when FC is informed by SC, the estimated networks become more resilient to noise and present a consistent topology and nodal strength under perturbations.
Human EEG Data
After validating the algorithm in benchmark data recorded in rats, we employed the si-STOK filter to model FC in event-related human EEG data. We modeled FC in a large-scale network of 68 brain regions in response to faces and scrambled stimuli (Desikan et al., 2006) (see Methods and Figure 3A). On the electrode level, the comparison of evoked responses between faces and scrambled images revealed the typical topography and time course of the face-related N170 component (faces minus scrambled, peak at 160 ms), with the largest difference between conditions localized in the right fusiform and nearby occipitotemporal areas (see Figure 3B).
Figure 3C shows the effect of incorporating SC priors on the estimated FC at a time window of interest around the N170 response. FC matrices obtained through the si-STOK filter showed the clear shrinkage of functional connections for weak and absent SC, leading to FC matrices that partly inherit the structure of SC but preserved intrinsic patterns of FC coupling (see Figure 3C), in line with the rat EEG benchmark results. For the same time window of evoked activity, we compared the summed outflow from each area between the two conditions (faces minus scrambled, summed MDI) as a measure of changes in nodal strength during face processing. In the ranked outflow, the two filters agreed in identifying the bilateral inferior temporal gyrus and the left fusiform gyrus as the three areas with the largest increase in outflow in response to faces (see Figure 3D). Without structural priors, however, frontal regions were also ranked among the largest drivers at short poststimulus latencies (e.g., rFPol, lFPol; see Supplementary File 2 for abbreviations), and the outflow increase in response to faces was more or less pronounced throughout the entire network. With the inclusion of structural priors, the 10 largest drivers of face-related activity were all located in the primary and secondary visual cortex, including the bilateral fusiform, lateral occipital cortex, lingual gyrus (e.g., V1), and regions in the temporal cortex (see Figure 3D), while the summed outflow from the rest of the network decreased progressively.
These comparisons suggest that the inclusion of SC priors refines the topology of FC networks and portrays the contribution of each node in a more physiologically plausible way. This feature can aid the identification of hubs and critical modules in large-scale FC analysis. A further important question is whether SC priors also affect the temporal dynamics of FC. We evaluated this aspect in a final analysis where we compared the estimated changes in directed influences in response to face and scrambled stimuli, time-locked to the stimulus onset (from −100 to 500 ms). For this analysis, we considered a subset of regions in the core face network: the right inferior temporal gyrus (rIT), the right fusiform (rFu), and the right superior temporal sulcus (rB). The functional role of these brain areas in face processing is well documented (Fox et al., 2009; Haxby et al., 2000), and their SC is a predictive feature of face-specific activity (Saygin et al., 2011), representing a functionally specialized module with known structure-function relationships. Figure 4 shows the estimated time-varying MDI with and without SC priors. This comparison revealed clear differences that extended well beyond the basic outflow summary described above, specifically the following: (a) SC priors led to quantitative increases in unidirectional interactions at specific latencies, consistent with face-related evoked dynamics (e.g., from rFu to rIT, from rIT to rB); (b) SC priors more clearly show the sequence of unidirectional interactions between nodes (e.g., from rIT to rFu, followed by rFu to rIT); (c) SC priors shrunk and underestimated weak FC for SC-absent connections, reducing the risk of false positives (e.g., the connection from rIT to rB at very short poststimulus latencies).
DISCUSSION
We introduced and validated a new adaptive filter for combining structural and dynamic functional connectivity, the si-STOK. The algorithm builds on a recent variant of the general linear Kalman filter (Kalman, 1960; Milde et al., 2010; Pascucci et al., 2020) and allows incorporating structural priors in a multivariate autoregressive modeling framework with high temporal resolution. Structural priors are used as starting assumptions on the structure of a regularizing matrix applied to the estimation of time-varying multivariate autoregressive models, in the framework of the generalized Tikhonov regularization method (Plato & Vainikko, 1990).
We tested the filter in rat epicranial recordings and human EEG data, using SC priors from a meta-analysis of tracer studies and DTI metrics, respectively. Compared with FC without SC priors, we found the following advantages. First, FC estimates were more consistent and more resilient to noise. Second, SC priors promoted sparser FC networks and favored a more accurate identification of the main network drivers at expected poststimulus latencies. Third, using SC as prior variance provided solutions with intrinsic protection against SC-related false negatives (for discordant SC-FC, the filter relied more on the data and less on the prior), and robustness against false positives (strong SC did not inflate FC unless supported by the data).
The first two aspects represent desired features considering the expected sparsity of FC networks and the sparse topology of the underlying structural links (Markov et al., 2012; Pagnotta et al., 2019; Valdés-Sosa et al., 2005). Previous work has shown how sparse and regularized approaches to FC analysis can decrease spurious connections, increase robustness to noise (Pagnotta et al., 2019), and counteract issues due to limited data points (Antonacci et al., 2019; Valdés-Sosa et al., 2005). Informing sparse solutions through the fixed topology of SC links has the additional benefit of introducing a biologically grounded space for regularization. This represents an advantage in conditions where regularization may have a strong impact on the model structure, such as under multicollinearity and nonindependence among time-series (e.g., under linear mixing of source EEG activity due to volume conduction; Anzolin et al., 2019; Haufe et al., 2013). The resulting FC partly inherits the sparsity and topological properties of the SC matrices, while preserving the strength and directedness of functional interactions. This may ultimately facilitate graph analysis of functional networks, such as the identification of FC hubs, modules, and nodal properties without additional sparsity-based and consensus-based thresholding, which may lead to unstable and threshold-dependent network estimates (Garrison et al., 2015).
The third important feature of the si-STOK is the protection against false positives due to invalid SC priors. Previous studies, including large-scale validations of tractography pipelines, have reported a high ratio of invalid connections and a substantial amount of false positives (Maier-Hein et al., 2017). This caveat undermines the possibility to simply mask or weight FC by SC, increasing the risk of inflating FC for invalid SC connections. The proposed algorithm employs the generalized Tikhonov method (Plato & Vainikko, 1990), a powerful and versatile regularization scheme under a Bayesian perspective where priors are combined with the observed data. Strong SC, therefore, does not necessarily inflate FC, as demonstrated in our test in benchmark data. Conversely, strong FC can still be detected in the absence of SC. This was also evident from the results in rat EEG data, where we observed FC between the primary somatosensory and the parietal cortex (e4 to e2; see Figure 2) even under a strong prior of no SC. Although reduced, FC between these areas was still larger compared with other connections for which SC was present but only weak FC was expected from physiology (e.g., from e4 to e8). This accommodates the possible divergence between FC and SC connections (Honey et al., 2009; Lim et al., 2019), which may arise from indirect structural connections, false negatives in SC (Damoiseaux & Greicius, 2009), or because of the differential engagement of specific functional modules under different task demands (Sokolov et al., 2019).
From our test in benchmark data it is also clear that, under certain circumstances, SC priors have minor effects on FC. This may occur, for instance, when using dense SC matrices in conditions where the signal-to-noise ratio is high, or when SC does not deviate drastically from FC. The rat EEG data analyzed here are an example. The rat cortex is essentially flat (lissencephalic) with few expected deep sources. The signal recorded at each electrode is therefore an accurate representation of the activity flowing through the structural pathways in the cortex underneath (Quairiaux et al., 2011). Using dense SC priors, generally in agreement with the expected FC, provided no additional or diverging information (see Figure 2B). In many other applications, however, signals are contaminated with multiple sources of noise and the recordings are not direct measurements of brain activity and connectivity. Under these common circumstances, SC priors may have more appreciable and beneficial effects on FC estimates. Our manipulation of added noise confirmed this advantage (Figure 2C).
Recently, a method to combine structural and functional connectivity in the framework of autoregressive modeling has been proposed and tested in fMRI data (Crimi et al., 2021). This approach relies on stationary (e.g., time-invariant) MVAR models in which a set of constraints, based on SC, force the model to estimate parameters only for existing, direct structural connections. The estimation is performed using a gradient descent approach. While our algorithm bears similarities to this approach, the key differences are that si-STOK is developed for time-varying MVAR modeling of fast, nonstationary dynamics, and that it incorporates structural information as regularizing priors, rather than strong constraints on model estimation. The approach presented here, therefore, should provide solutions that are more robust against SC false negatives, as SC provides information about the variance of FC, rather than imposing the presence/absence of a connection.
The proposed algorithm is the first to integrate SC in a directed and dynamic measure of FC. We evaluated the effect of SC priors on large-scale dynamics of directed interactions in human source EEG activity during a face detection task. We considered dynamic interactions in a subset of regions corresponding to key nodes of the core face network (Haxby et al., 2000; Saygin et al., 2011). Our results showed that SC priors can also shape the temporal dynamics of FC. Particularly, incorporating SC priors revealed an initial face-selective increase of FC from the right inferior temporal gyrus to the fusiform, followed by an increase of FC from the fusiform to the inferior temporal gyrus and from the inferior temporal gyrus to the superior temporal sulcus. Without SC priors, a precise sequence of FC changes was less distinguishable, and face-evoked increases in FC were also present at unreasonably short poststimulus latencies (e.g., from the inferior temporal gyrus to the superior temporal sulcus; see Figure 4). The observed pattern may well reflect the buildup of face-specific processing supported by a hierarchy of recurrent interactions in the core face network: the inferior temporal cortex may first relay information about global aspects (e.g., the shape and coarse structure of the face stimuli used here) to the fusiform which then feed back information about finer details (Goffaux et al., 2011; Sugase-Miyamoto et al., 2011; Tovée, 1995). Although the interpretation of these results remains speculative and goes beyond the purpose of the current work, the results of this analysis are a clear example of how SC priors can enhance or downregulate time-varying dynamics in FC.
Our adaptive filter follows the aim of recent biological and mathematical modeling approaches that use anatomical information for predictive and generative models of functional interactions (Crimi et al., 2021; Jirsa et al., 2010). Particularly, structural graphs have been used as priors in the Bayesian framework of dynamic causal modeling (Sokolov et al., 2019). In this context, SC represents a prior on the generation of neuronal dynamics and interregional coupling, with the target of identifying candidate mechanisms that support the biophysical transfer of neuronal activity. By contrast, the si-STOK comes from the framework of Granger causality (Granger, 1969), in which causal influences are described through statistical dependencies between the past and present of multivariate time series. Hence, the target property is the effect and not the mechanism. The use of SC information has therefore a different meaning than in a generative framework. SC priors act as constraints on the estimation of “effects”—linear and directed statistical dependencies between signals recorded at multiple brain areas. While similar priors could in principle be used with dynamic causal models (Sokolov et al., 2019), the MVAR-based si-STOK formulation is computationally more advantageous for large networks. Si-STOK thus provides an efficient way to exploit structural constraints in the estimation of large-scale, high-temporal-resolution FC, building on an algorithm suited to track nonstationary signals with rapid and abrupt changes, such as those recorded during evoked brain activity (Pascucci et al., 2020).
As a final note, it is worth mentioning that FC, in general, refers to a statistical covariation between two signals and, therefore, FC can also exist without a structural link, as reported in many studies (Reid et al., 2019). The measure proposed here incorporates the physiologically grounded assumption that direct and directed statistical influences must occur between nodes of a network that are structurally linked (e.g., not as the result of indirect and mediating variables).
In sum, we provide a new method for dynamic FC that incorporates priors on structural connectivity for the analysis of multivariate electrophysiological signals. The algorithm offers a simple and powerful tool for multimodal imaging that can meaningfully contribute to integrative approaches in network neuroscience (Crimi et al., 2016; Lei et al., 2015; Sokolov et al., 2019; Stephan et al., 2009). It allows the incorporation of various types of SC, weighted or binary, symmetric or directed. Because of its simple form, the algorithm can flexibly incorporate different types of SC priors, from basic metrics, such as the number of white matter fibers and the Euclidean distance, to graph-derived metrics, such as the path length and communicability (Vázquez-Rodríguez et al., 2019), as well as FC priors from other modalities.
METHODS
Benchmark EEG Data
Rat EEG recordings.
Benchmark data are publicly available EEG recordings from a grid of 16 stainless steel electrodes placed directly on the skull bone of 10 young Wistar rats (P21; half males). Data were collected during unilateral whisker stimulations under light isoflurane anesthesia (available from https://osf.io/fd5ru). Details about the recording can be found in the original publication (Plomp et al., 2014; Quairiaux et al., 2011). Data were acquired at 2,000 Hz, bandpass filtered online, and downsampled to 1,000 Hz before connectivity analysis. All animal handling procedures were approved by the Office Vétérinaire Cantonal (Geneva, Switzerland) following Swiss federal laws.
Rat structural connectome.
Structural priors for the rat EEG benchmark were obtained from a published meta-analysis of histologically defined axonal connections between cortical regions in rats (Bota et al., 2015; Swanson et al., 2017). Particularly, we used one dataset containing ranked connection weights based on reported association and commissural connections. Details of the dataset can be found in the original publication (Dataset S3; from Swanson et al., 2017). Ranked connection weights for pairs of regions corresponding to the electrodes recording sites were manually selected by an expert biologist from primary visual areas, somatosensory, primary, and secondary motor areas, and cingulate cortex (see Supplementary File 1). Connection weights, ranging from 0 to 12 (from absent to very strong; see Supplementary File 1) were organized into a 16-by-16 structural connection matrix whose main diagonal elements (e.g., self-connections) were set to the maximum value of 12. The structural matrix was then normalized to the maximum value and used as a prior for the time-varying connectivity analyses of the rat EEG data.
Human EEG Data
Task and stimuli.
EEG data were recorded while 20 participants (3 males, mean age = 23 ± 3.5) performed a face detection task (see Figure 3A) in a dimly lit and electrically shielded room. Each trial lasted 1.2 s and started with a blank screen (500 ms). After the blank screen, one image (either a face or a scrambled image of a face) was presented for 200 ms, and participants had the remaining 1,000 ms to respond. The task was to report whether they saw a face or not (yes/no task) by pressing two buttons in a response box with their right hand (ResponsePixx, VPixx technologies). Faces and scrambled faces were randomly interleaved across trials. After the response and a random interval (from 600 to 900 ms), a new trial began. The experiment consisted of four blocks of 150 trials each, for a total of 600 trials, that is, 300 with faces and 300 with scrambled faces. Face stimuli were female and male faces (4 by 4 degrees of visual angle, dva) taken from online repositories and cropped with a Gaussian kernel to smooth the borders. Scrambled images were obtained by fully randomizing the phase spectra of the original images (Ales et al., 2012). Stimuli were generated using PsychoPy (Peirce, 2008) and presented on a VIEWPixx/3D display system (1920 × 1080 pixels, refresh rate of 100 Hz). All participants provided written informed consent before the experiment and had a normal or corrected-to-normal vision. The experiment was approved by the local ethical committee.
EEG acquisition and preprocessing.
Data were recorded at 2,048 Hz with a 128-channel Biosemi Active Two EEG system (Biosemi, Amsterdam, the Netherlands). Signal quality was ensured by monitoring and maintaining the offset between the active electrodes and the common mode sense–driven right leg (CMS-DRL) feedback loop under a standard value of ±20 mV. After each recording session, individual 3D electrode positions were digitized using an ultrasound motion capture system (Zebris Medical GmbH). One participant was excluded because of too many motion artifacts, leaving 19 datasets for analysis. Further details on the recordings and preprocessing pipeline can be found in the original manuscript of the VEPCON dataset (OpenNeuro Dataset ds003505; Pascucci et al., 2021).
EEG source imaging.
EEG source imaging was performed using Cartool (Brunet et al., 2011) and custom-made scripts in MATLAB R2020b (9.9.0.1524771 Update 2). Source reconstruction was based on individual MRI data and the LAURA algorithm implemented in Cartool (regularization 6; spherical model with anatomical constraints, LSMAC), limiting the solution space to gray matter voxels. Source activity for freely oriented dipoles was extracted from all the source points inside each of the 68 cortical areas and projected to a representative single direction for each area, using the singular values decomposition approach (Rubega et al., 2019; time window for estimating the main direction: 140–250 ms poststimulus). Before functional connectivity analysis, a global z-score transformation was applied to the entire dataset of each participant. Epochs of source activity corresponding to trials with behavioral errors were then removed and the dataset was divided into two conditions, according to trials containing faces or scrambled stimuli.
MRI acquisition and preprocessing.
A detailed description of the MR acquisition and preprocessing can be found in the original manuscript of the VEPCON dataset (OpenNeuro Dataset ds003505; Pascucci et al., 2021).
Structural connectome.
Structural connectivity matrices were estimated from the reconstructed fiber orientation distribution (FOD) image using the SD_stream deterministic streamline tractography algorithm implemented in MRtrix 3.0.0-RC1 (Tournier et al., 2019). Fiber streamline reconstruction started from seeds in the white matter that were spatially random, and the whole process was completed when 1M fiber streamlines were reconstructed. At each streamline step of 0.5 mm, the local FOD was sampled, and from the current streamline tangent orientation, the orientation of the nearest FOD amplitude peak was estimated via a Newton optimization on the sphere. Fibers were stopped if a change in direction was greater than 45 degrees. Fibers with a length not in the 5–200 mm range were discarded. The streamline reconstruction process was complete when both ends of the fiber left the white matter mask. Then, for each scale, the parcellation was projected to the native DTI space after symmetric diffeomorphic coregistration between the T1w scan and the diffusion-free B0 using ANTs 2.2.0. Finally, the connectivity matrix was built according to the Desikan parcellation atlas, using the log of the number of fibers as the connectivity measure. Only 68 areas from cortical volumes were included in the structural matrix and used for functional connectivity analysis. A consensus group-representative structural brain connectivity matrix was generated from the connectomes of all participants’ connectomes using the method introduced in Betzel et al. (2019). Each participant connectivity matrix was then thresholded by preserving the group-representative connection density independently for intra- and interhemispheric connections. This allows retaining more interhemispheric connections in comparison to simple connectome thresholding. The resulting connection density is set to 30%. The median of the obtained structural connectomes across participants was then normalized to its maximum and used as a group structural prior for connectivity analysis.
Adaptive Filtering
Self-tuning optimized Kalman filter (STOK).
Structural priors.
Although this approach is deterministic, it has a natural Bayesian interpretation: Equation 6 is equivalent to expressing a prior belief on the functional connections bi←j entering each channel, under a multivariate normal distribution N(bi←j; x0, Qi←j). This regularization scheme requires the nontrivial conversion of SC to FC priors. A commonly employed strategy is to set the prior expectations on x0 to zero (e.g., no functional connectivity; Sokolov et al., 2019; Stephan et al., 2009), and to define the prior variance Qi←j based on the strength of SC. Under the mild assumption of a positive and monotonic relationship between structure and function, strong SC can be translated into large FC prior variance , corresponding to small regularization values in that favor nonzero FC when supported by the data. Conversely, weak SC can be translated into small prior variance, increasing the effect of regularization, and shrinking the tv-MVAR coefficients toward their expected value of zero. Mapping SC to FC priors also requires the scaling of SC to a range of suitable values, given that regularization acts on the magnitude and scale of autoregressive coefficients. Normalized SC values are in the 0–1 range. For the sake of the present work, we scaled SC from 10−4 to 0.1, a range that produced clear effects of regularization for both rat and human EEG data, without an excessive shrinking of all the coefficients. This range was chosen ad hoc, by varying the maximum of the scaling range and selecting a value for which (a) the correlation between SC and FC increased, and (b) the correlation between STOK (noninformed by SC) and si-STOK estimates would not drastically decrease. Note that the choice of smaller scaling maxima reduces the regularization effects and provides estimates that approximate the STOK results. The choice of a larger scaling maxima increases the effect of regularization, introducing an excessive shrinkage of the coefficients. An alternative, data-driven method to select the scaling maxima is presented as Supplementary File 3.
Time-Varying Directed Functional Connectivity
Directed connectivity measure.
Rat EEG functional connectivity.
We tested the new algorithm and compared the performance against the regular STOK using benchmark EEG data from epicranial recordings in rats. These data provide a good benchmark for time-varying connectivity analysis because of the known structural and functional connectivity patterns. After whisker stimulation, the evoked FC is expected to follow the underlying SC, with the activity that propagates from the contralateral primary somatosensory cortex to nearby parietal and frontal regions, at short latencies (5–25 ms poststimulation). This pattern was extensively validated in previous work (Pagnotta & Plomp, 2018; Pascucci et al., 2020; Plomp et al., 2014).
As a proof of concept, we first verified the effect of adding structural priors with different levels of sparsity. Two SC matrices were used as priors for the si-STOK, one obtained from the original SC (see Rat structural connectome in the Methods section and Supplementary File 1), the other obtained by applying proportional thresholding to the same SC, retaining only 25% of the strongest connections, setting the remaining to zero and the diagonal (self-self connections) to one. The MDI metric was derived from tv-MVAR models with a model order of 4 (Pagnotta & Plomp, 2018; Pascucci et al., 2020). In the comparison, we focused on the outflow from the contralateral somatosensory cortex to the rest of the network, at the peak latency. The peak latency was estimated from the results of the regular STOK as 13 ms poststimulus, in line with previous reports (Pascucci et al., 2020; Plomp et al., 2014).
In a second step, we compared the performance of the two filters under noise perturbation. We mixed the original data with white noise signals of the same size as the data and with an amplitude corresponding to the 95th percentile of the data. We varied the mixing ratio between the original data and the noise signals in five levels (from 0 to 0.5, in steps of 0.1; 0 = 100% of the data and 0% of the noise). The noise at each level was regenerated 30 times. For each iteration, we estimated (a) the difference between the outflow from the contralateral somatosensory cortex and the average outflow of the network, at peak latency; and (b) a binary, directed adjacency matrix preserving 50% of the strongest connections at peak latency. From the first measure, we estimated the difference between the outflow from the primary somatosensory cortex and the network average outflow, across animals—that is, the ability of the two algorithms to discriminate the somatosensory cortex as the main driver under noise perturbations. From the second measure, we derived a metric of consistency of the estimated networks under increasing noise. The consistency was obtained as the proportion of binary connections in the adjacency matrix that, for each level of noise larger than 0, were identical to those estimated in the absence of noise—that is, the consistency of the estimated strongest connections under increasing noise. For both measures, the zero-noise level was used as a baseline.
Human EEG directed functional connectivity.
In the analysis of human EEG data, we evaluated the results of the STOK and si-STOK filter in estimating large-scale FC during face processing (see Figure 3A). For both filters, we used a model order of 10, in line with values used before (Pascucci et al., 2018; Pascucci et al., 2020). We first compared evoked activity after face and scrambled stimuli at the scalp and source level (see Figure 3B). We selected a time window centered on the scalp and the source N170 peak component (140–180 ms) as the latency of interest for FC results. In the N170 window, we evaluated the estimated difference in network MDI across conditions. For this difference, we compared the summed outflow obtained with the two filters, from each node of the 68-area network (see Figure 3D). In a subsequent analysis, we focused on dynamic directed interactions among a subset of areas known to be involved in face processing (e.g., regions of the core face network; Haxby et al., 2000). We considered three areas, all in the right hemisphere: the fusiform gyrus (rFu), the inferior temporal gyrus (rIT), and the superior temporal sulcus (rB; see Figure 4). We then compared directed influences among each pair of areas in response to face or scrambled stimuli across time. Significance was assessed using group-permutation statistics, as the proportion of group-averaged MDI differences that were larger or smaller than the observed ones, after shuffling the sign of the difference across participants 100,000 times. An uncorrected p value (p < 0.01) was used as the threshold, with the exploratory aim to qualitatively compare the results with and without the inclusion of SC priors.
ACKNOWLEDGMENTS
The authors thank Guru Prasad Padmasola and Charles Quairiaux for providing guidelines and useful material for the analysis of the rat benchmark data, and Larry W. Swanson and Joel Hahn for providing the rat structural connectivity dataset.
SUPPORTING INFORMATION
Supporting information for this article is available at https://doi.org/10.1162/netn_a_00218.
AUTHOR CONTRIBUTIONS
David Pascucci: Conceptualization; Data curation; Formal analysis; Investigation; Methodology; Software; Validation; Visualization; Writing – original draft. Maria Rubega: Conceptualization; Investigation; Methodology; Software; Validation; Writing – review & editing. Joan Rué-Queralt: Data curation; Methodology; Resources; Software; Writing – review & editing. Sebastien Tourbier: Formal analysis; Methodology; Resources; Software. Patric Hagmann: Conceptualization; Funding acquisition; Methodology; Project administration; Resources; Supervision; Writing – review & editing. Gijs Plomp: Conceptualization; Funding acquisition; Investigation; Project administration; Resources; Supervision; Writing – original draft; Writing – review & editing.
FUNDING INFORMATION
Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (https://dx.doi.org/10.13039/501100001711), Award ID: PP00P1_183714. Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (https://dx.doi.org/10.13039/501100001711), Award ID: PP00P1_190065. Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (https://dx.doi.org/10.13039/501100001711), Award ID: PZ00P1_179988. Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (https://dx.doi.org/10.13039/501100001711), Award ID: CRSII5-170873.
TECHNICAL TERMS
- Dynamic functional connectivity:
A loose term to define functional connectivity measures that are functions of time, accounting for temporal changes and reconfigurations of functional network patterns.
- Direct connectivity:
A metric that distinguishes between direct and indirect flows in a network, quantifying the influence that one signal produces directly on another, without including indirect paths.
- Directed connectivity:
A directional measure of statistical dependence among multiple signals, quantifying the influence directed from one signal to another in the Granger causality framework.
- Time-varying multivariate autoregressive model:
A model that estimates lagged linear effects within a multivariate system of variables, providing recursive estimates that are a function of time and are collected for each time point.
- Linear adaptive filter:
A linear filter that self-adjusts its transfer function and coefficients over time, according to an optimization rule. In the domain of functional brain networks, adaptive filters adapt to the time-varying properties of neuronal signals.
REFERENCES
Supporting Information
Author notes
Competing Interests: The authors have declared that no competing interests exist.
Handling Editor: Olaf Sporns