Learning to pronounce a foreign phoneme requires an individual to acquire a motor program that enables the reproduction of the new acoustic target sound. This process is largely based on the use of auditory feedback to detect pronunciation errors to adjust vocalization. While early auditory evoked neural activity underlies automatic detection and adaptation to vocalization errors, little is known about the neural correlates of acquiring novel speech targets. To investigate the neural processes that mediate the learning of foreign phoneme pronunciation, we recorded event-related potentials when participants (N = 19) pronounced native or foreign phonemes. Behavioral results indicated that the participants’ pronunciation of the foreign phoneme improved during the experiment. Early auditory responses (N1 and P2 waves, approximately 85–290 ms after the sound onset) revealed no differences between foreign and native phonemes. In contrast, the amplitude of the frontocentrally distributed late slow wave (LSW, 320–440 ms) was modulated by the pronunciation of the foreign phonemes, and the effect changed during the experiment, paralleling the improvement in pronunciation. These results suggest that the LSW may reflect higher-order monitoring processes that signal successful pronunciation and help learn novel phonemes.
When learning to pronounce foreign phonemes, the individual needs to evaluate how well the sound they produced matches the target phoneme and, in case of a mismatch, attempt to improve their phonation. Such learning may be based on fast and automatic unconscious speech control processes, but it may also depend on later processes that are associated with the conscious evaluation of phonation. However, relatively little is known about the neural mechanisms that underlie the learning of foreign phoneme production. In this study, by comparing electroencephalographic (EEG) activity evoked by self-produced and passively heard foreign and native phonemes, we investigate which auditory evoked potentials correlate with learning to pronounce foreign phonemes.
Previous research on the neural processing of foreign phonemes has focused on examining changes in auditory evoked activity elicited by passively heard sounds. Studies employing the oddball paradigm have shown that foreign phonemes elicit weaker mismatch negativity responses than native phonemes (Díaz et al., 2008; Näätänen et al., 1997; Peltola et al., 2003), indicating a poorer ability to discriminate foreign phonemes. Training improves the discrimination of phonemes, and this is reflected in the mismatch response (Tamminen et al., 2015; Tremblay et al., 1998). Phoneme discrimination training also increases the amplitude of early (N1/P2) auditory event-related potentials (ERPs), which has been interpreted as training-induced neuroplastic changes in the auditory cortex (Alain et al., 2007; Reinke et al., 2003; Saloranta et al., 2020). Additionally, studies have shown that the amplitude of a late slow wave (LSW; 300–500 ms) to passively heard phonemes correlates with phonemic learning (Alain et al., 2007; Reinke et al., 2003; Saloranta et al., 2020), but the functional role of this correlate is unclear.
Learning to discriminate the acoustic features of passively heard foreign phonemes is clearly important for learning to produce the phoneme. However, to accurately vocalize novel phonemes one needs to also learn the motor commands that enable reproduction of the desired acoustic features. The process of learning to produce novel phonemes involves tracking the auditory feedback of one’s own vocalizations to adjust future pronunciation. Studies in which speech is artificially altered (e.g., by shifting pitch) indicate that individuals can adjust their speech based on auditory feedback in just 100–150 ms (Hain et al., 2000; Houde & Jordan, 1998). This type of rapid feedback control relies on “efferent copies” of motor commands that the motor system relays to the auditory cortex to compare how well the produced speech matches the targeted motor commands (Hickok et al., 2011; Houde & Nagarajan, 2011; Tourville & Guenther, 2011). Because of this interplay between motor and sensory systems, the neural processing of passively heard and self-produced sounds differs significantly (Curio et al., 2000; Houde et al., 2002). Therefore, to understand how individuals learn novel phonemic targets, it is crucial to examine electrophysiological responses to self-produced sounds.
The electrophysiological correlate of the feedback control of self-produced speech is the suppression of auditory evoked activity. Auditory ERPs in response to self-produced sounds are suppressed in the N1 (100–200 ms) and the P2 (200–300 ms) time windows, as compared to when the same sounds are passively heard (Behroozmand et al., 2011; Curio et al., 2000; Heinks-Maldonado et al., 2005; Houde et al., 2002; Railo et al., 2020). This phenomenon is known as the speaking-induced suppression (SIS). Using magnetoencephalography, Niziolek et al. (2013) observed that the amplitude of SIS (i.e., amplitude difference between self-produced and passively heard sounds) tracked variation in phonation: Auditory responses to phonemes that deviated from prototypical phonemes produced a decreased SIS, and the size of SIS predicted corrections to vocalization. Similarly, when speech is artificially altered, SIS decreases, suggesting that the brain detects a mismatch between feedforward motor commands and heard speech (Behroozmand et al., 2009; Behroozmand & Larson, 2011; Chang et al., 2013).
In addition to enabling online adjustments to pronunciation, such a feedback control mechanism may contribute to the process of learning to correctly pronounce foreign phonemes: the individual learns to better produce the desired sound based on errors in previous vocalizations. A similar mechanism is assumed to contribute to phonemic learning in children during speech acquisition. After learning the target sound, the motor commands used to produce the sound are fine-tuned based on feedback control (Tourville & Guenther, 2011). However, we are not aware of any studies that have examined whether SIS amplitude tracks the process of learning to produce a novel foreign phoneme. Many studies have investigated sensorimotor adaptation of speech to altered auditory feedback, but such learning effects are relatively short-lived and represent changes to motor programs the individual already knows (e.g., Houde & Jordan, 1998; Lametti et al., 2018). In contrast, when learning a novel phoneme, the individual needs to learn completely novel sensorimotor speech targets.
Online adjustments to speech are typically considered rapid low-level processes, but the processes of learning to pronounce foreign phonemes may also depend on higher-order processes that take more time to unfold. SIS typically refers to effects observed in the N1 and P2 time windows, but the comparison of actively spoken and passively heard phonemes sometimes also reveals differences in later time windows where a LSW (300–500 ms) is often observed in speech tasks. Differences in this late time window are often neglected, likely because they are too late to reflect automatic feedback speech control, but also because there is no theoretical framework within which to interpret these findings. Whereas the classical SIS time windows (N1, P2) likely corresponds to the automatic preconscious evaluation of self-produced speech, the later time windows may correspond to attentive evaluation of pronunciation. Because the attentive evaluation of self-produced speech is likely to contribute to phonemic learning, and because earlier studies suggest that LSW may correlate with phoneme discrimination learning (Alain et al., 2007; Reinke et al., 2003; Saloranta et al., 2020), it is important to investigate not only the earlier time windows (N1 and P2) but also the LSW.
Here, we recorded auditory ERPs when Finnish participants reproduced a native /ö/ phoneme or a foreign Estonian /õ/ phoneme (Speak condition) after hearing the target spoken by a native speaker. In a control condition, the participants listened to a recording of the phoneme they had just spoken (Listen condition). Comparison of the Speak and Listen conditions corresponds to the SIS, and it also allows us to test whether possible associations between pronunciation and ERP amplitudes reflect active vocalization or the passive processing of acoustic information. Assuming that participants are not able to produce the foreign phoneme as accurately as the native phoneme, we expected to observe a smaller suppression of N1 amplitudes to self-produced foreign phonemes, as compared to passively heard phonemes (i.e., reduced SIS, indicating that the auditory system detects a mismatch between produced and attempted sounds). Furthermore, assuming that participants learn to better pronounce the foreign phoneme during the experiment, we analyzed the trial-by-trial changes in ERP amplitudes in three time windows (N1, P2, and LSW) to test whether changes in ERP amplitudes track improvements in pronunciation.
MATERIALS AND METHODS
Twenty-one participants (students at the University of Turku) volunteered for this study. All participants were Finnish, with normal hearing and with no diagnosed learning disabilities or neurological disorders. All participants were monolingual and reported no previous experience in learning Estonian. Two participants were excluded from statistical analyses due to excessive noise in EEG. Thus, the final sample included 19 participants (range 18–35 years; 17 females, 2 males). The study was conducted according to the principles of the Declaration of Helsinki. All participants provided informed consent to participate in the study. The experiment was approved by the Ethics Committee for Human Sciences at the University of Turku.
Stimuli and Procedure
An overview of a single experimental trial is presented in Figure 1A. Participants heard a recording of the Estonian phoneme /õ/, or the Finnish phoneme /ö/, in a random order. We call this the Cue stimulus condition. The phoneme /õ/ is not part of the Finnish phonological system and was therefore unfamiliar to the subjects. Acoustically, it resembles the Finnish phoneme /ö/ (e.g., Näätänen et al., 1997). After hearing the Cue phoneme, participants attempted to repeat it as well as possible (Speak condition). After repeating the sound, they heard a playback of their produced phoneme (Listen condition). The stimuli were separated by approximately a 2–3 s interval. Intertrial interval was about 4 s. This process was repeated 50 times in a block, and the experiment consisted of five blocks (250 repetitions in total during the experiment). There was a 2–5 min break between blocks.
Cue stimuli (/ö/ and /õ/) were recorded by the same native Estonian (female) as both phonemes are part of the Estonian language. The two phonemes were approximately the same amplitude, pitch, and duration (500 ms). To examine how Finnish individuals hear the stimuli, we asked 21 participants (age range 20–50, 13 females; different participants than those who took part in the main study reported in this article) to listen to the stimuli, to identify the phoneme, and to rate how confident (scale 1–4) they were in their response. Twenty out of the 21 participants correctly identified the native phoneme as /ö/ with high confidence (M = 3.33, SD = 0.86). None of the participants correctly identified the Estonian /õ/ phoneme: Fourteen participants identified it as Finnish /ö/ (confidence M = 3.14, SD = 0.77), two participants identified the phoneme as Finnish /y/ (M = 2.0, SD = 0), two as the Russian ы (M = 3.50, 0.70), two as the Swedish å (M = 4, SD = 0), and one participant believed the phoneme corresponded to the pronunciation of the German ü (confidence = 4). In sum, this result shows, that Finnish individuals recognize the native phoneme, but misidentify the Estonian phoneme.
The Cue and Listen stimuli were played to the participants from two TEAC LS-X8 speakers placed about 1 meter to left and right of the participant. Participants’ pronunciations were recorded using a Trust GXT 242 Lance microphone and saved in wave file format.
EEG was recorded with 32 passive electrodes (EASYCAP, 2022) placed according to the 10–10 electrode system (Nuwer et al., 1998). Surface electromyograms were measured with two electrodes above and below the lips, and below and to the side of the right eye. Reference electrode was placed on the nose. Ground electrode was placed on the forehead. EEG was recorded with a NeurOne Tesla amplifier using Version 18.104.22.168 software (Bittium, 2022). Sampling rate was 500 Hz. In addition, the auditory stimuli (Cue, Speak, and Listen conditions) were recorded as EEG signals using a microphone. This allowed us to accurately mark the onset times of auditory stimuli on the EEG.
EEG was processed using EEGLAB Version 14.1.1 software (Delorme & Makeig, 2004) in MathWorks’s Matlab 2014b. First, we high-pass filtered the microphone signals recorded with EEG at 100 Hz (to remove noise but keep the sound signal and its transient onset) and used it to add markers of the onsets of the stimuli on the continuous EEG data. The onset of auditory stimuli was determined automatically as follows. The microphone signal had to remain above a specified amplitude threshold for 10 consecutive samples, and then a marker was added to the sample where the threshold was first crossed. As shown in Figure 1B, this procedure yielded accurate estimates of phoneme onset times in all three experimental conditions. After this, the microphone channels were removed from the data.
We rejected channels containing artifacts using EEGLAB’s pop_rejchan function based on kurtosis, spectrum, and probability measures. Then, data were 1 Hz high-pass filtered (Klug & Gramann, 2020) using pop_eegfiltnew function, and 50 Hz line noise was reduced using the ZapLine plugin (de Cheveigné, 2020). We used artifact substance reconstruction to clean continuous EEG using a cutoff parameter at 20 (Chang et al., 2020). We then average-referenced the data and ran independent component analysis (ICA) using the extended infomax algorithm. After the ICA, we used the DIPFIT plug-in (SCCN, 2019) for localizing equivalent dipole locations of the independent components. The rejection threshold was set at 100 (no dipoles were rejected) and two dipoles constrain in symmetry. We used IClabel to automatically categorize components into brain-based and various non–brain-based categories (Pion-Tonachini et al., 2019). Components with residual variance <15%, and >70% probability that the component is brain based, were not considered artifactual (i.e., other components were removed). After this, the removed channels were interpolated.
Next, the data were low-pass filtered at 40 Hz and cut into segments starting 200 ms before stimulus onset and ending 600 ms after stimulus onset. Artifactual trials were removed using the pop_jointprob function (local and global thresholds = 3). The average number of trials per participant in the Finnish Cue condition was 97 (median = 99.5, SD = 9.1) and in the Estonian Cue condition 109 (median = 112, SD = 10.1). The average number of trials in the Finnish Speak condition was 75 (median = 78, SD = 21.0) and in the Estonian Speak condition 88 (median = 99.5, SD = 28.3). In the Finnish Listen condition, the average trial number was 85 (median = 87, SD = 14.4) and in the Estonian Listen condition 95 (median = 98.5, SD = 16.4).
We used mixed-effects linear regression analysis to test if Condition (Speak vs. Listen) and Phoneme (Native /ö/ vs. Foreign /õ/) factors influenced ERPs at N1, P2, and LSW time windows in single-trial data. The benefit of mixed-effects models is that analysis can be performed on single-trial data while taking into account variation between participants. The analyses were performed on afrontal-central electrode cluster (average of amplitudes across electrodes F3, Fz, F4, FC1, FC2, C3, Cz, and C4, shown in Figure 1C). The analysis was run in Matlab 2014b. The Listen condition and Native phoneme were set as reference categories in the regression models (i.e., intercept is the Native phoneme in the Listen condition). In addition, trial number was included in the model as a continuous regressor, because we were interested in examining if ERP amplitudes changed as the experiment progressed (possibly due to learning). Each condition had its own running trial number (e.g., trial number 10 in Speak/Foreign indicated trial 10 in this specific condition). The trial number regressor was z scored (i.e., intercept of the model represents responses around experiment midpoint). The model included all the three predictors and their interactions as fixed-effects regressors (i.e., Condition × Phoneme × Trial number). The model included the intercept and Speak/Listen conditions as participant-wise random effects because more complex random effect structures (based on Akaike and Bayesian information criteria) led to inferior models. Separate models were fitted for each ERP component (N1, P2, and LSW, as described below), and the models were pruned by removing outliers.
To examine participants’ ability to pronounce the foreign phoneme, recordings of the participants’ pronunciation were rated by two native Estonians (one of the raters was co-author P. S.). During the rating procedure, the recordings were presented in random order, one participant at a time. Participants’ pronunciation was rated on a scale from 1 to 4. Rating 1 denoted vocalizations that did not resemble /õ/ at all (e.g., the participant vocalized the phoneme /ö/). Rating 2 denoted poor vocalizations that had a little resemblance to /õ/, but the produced phoneme still clearly deviated from the target. Rating 3 denoted good vocalizations that clearly resembled /õ/ without capturing the sound perfectly. Rating 4 corresponded to an excellent pronunciation of /õ/ (i.e., the pronunciation resembled a native speaker’s pronunciation of the phoneme). After examining interrater correlations (Figure 2C), the two ratings were averaged for statistical analyses. Pronunciation of native phonemes was not rated because, being part of Finnish phonetics, we assumed that participants have no difficulties in pronouncing these.
The aim of the ratings was to obtain a numerical index reflecting the external validity of pronunciation. To verify that the ratings reflect acoustic parameters of the target phoneme, we examined how well the frequency of the two first formants of participants’ vocalizations predicted the (averaged) ratings. The first and second formats of the participants’ single-trial vocalizations were estimated from the wave files using linear predictive coding in Matlab.
Figure 2A and 2B show phoneme plots of participants’ native and foreign phoneme pronunciations, respectively. Consistent with previous reports, pronunciation of the two phonemes differ especially in F2 (e.g., Asu & Teras, 2009; Näätänen et al., 1997). The ratings of the two native Estonian speakers moderately correlated with each other (rho = 0.54; linearly weighed Cohen’s kappa = 0.19; Figure 2C) but suggested that the participants had, in general, difficulties in pronouncing the foreign phoneme (mean rating = 2.46, SD = 0.81). To verify that the ratings captured systematic variation in the acoustics of the foreign phoneme, we used a linear mixed-effects model to examine how well the F1 and F2 frequencies predicted the ratings. The model included F1 and F2 frequencies and their interaction as fixed-effects predictors, and the same regressors and the intercept were included as by-participant random effects variables. The results indicated that F1 frequency (t = 1.97, p = 0.04), F2 frequency (t = 4.06, p < 0.001), and their interaction (t = 2.83, p = 0.004) predicted roughly 67% (adjusted R2) of variation in the ratings.
An additional linear mixed-effects model indicated that, on average, ratings of participants’ pronunciation of the foreign phoneme improved as a function of trials (β = 0.0014, t = 2.87, p = 0.004; the model included random, by-participant variation in the intercept and trial number regressor). This result is visualized in Figure 2E, which shows the model results for individual participants (thin gray lines) in addition to the group average (red line). Finally, to verify that the increase in ratings as a function of trials reflected true changes in the acoustic features of pronunciation, we calculated the Euclidean distance of single-trial foreign phoneme vocalizations from the “optimal” F1 and F2 formant combination (see white arrow in Figure 2D for visualization). The Linear mixed-effects model with trial number as a predictor indicated that formants approached “optimal” as a function of trial (t = −2.60; random-effect structure included by-participant intercept and trial number).
Butterfly plots of the ERPs in different experimental conditions are shown in Figure 1C. In both the Cue and Listen conditions, prominent N1 (negative peak at 160 ms) and P2 (positive peak at 250 ms) waves were observed. In addition, the LSW was observed around 350–500 ms after stimulus onset. As expected, the amplitude of the ERPs was suppressed in the Speak condition relative to the Listen condition, indicating SIS. In addition, the peak latency of the N1 and P2 waves were earlier and the scalp topography somewhat different in the Speak condition (as compared to the Listen and Cue conditions).
The critical comparison between the Listen and Speak conditions is visualized in Figure 3. Scalp distributions of the difference between the Listen and Speak conditions—reflecting SIS—are shown in Figure 3B, separately for the Native and Foreign phoneme conditions. We statistically analyzed how the experimental manipulations influenced the N1 (150–200 ms), P2 (230–290 ms), and LSW (320–440 ms) amplitudes. Based on the scalp maps of prominent waves (see Figure 1C), statistical analyses were performed on the frontal-central electrode cluster (F3, Fz, F4, FC1, FC2, C3, Cz, C4).
The results of the linear mixed-effects regression analyses on N1 amplitudes are presented in Table 1. The intercept represents the average N1 amplitude in the Listen/Native condition. The effect of Trial shows that the amplitude does not change statistically significantly (p = 0.17) as a function of trial. The main effect of the Speak condition indicates that, on average, amplitudes were 1.94 μV more positive in the Speak condition than in the Listen condition (p < 0.001), reflecting the SIS. This effect did not change statistically significantly during the experiment (Trial:Speak interaction, p = 0.67). N1 amplitudes to foreign phonemes were, on average, amplified by 0.38 μV (p < 0.001). The lack of Speak:Foreign interaction (p = 0.63) indicates that the SIS did not differ between the Foreign and Native conditions. Finally, the lack of a three-way Trial:Speak:Foreign interaction indicates that the SIS in the Foreign condition was not modulated by trial number (p = 0.88). The results of the model did not change markedly if the model was pruned by removing the (largely redundant) Trial regressor.
|Name .||Estimate .||SE .||t .||p .||Lower 95% CI .||Upper 95% CI .|
|Name .||Estimate .||SE .||t .||p .||Lower 95% CI .||Upper 95% CI .|
Because the latency of the N1 wave peaked earlier in the Speak than in the Listen condition, we repeated the analysis with the N1 amplitudes between 84–104 ms in the Speak condition. The overall pattern of results was similar to that reported in Table 1.
The results of the analyses regarding the P2 time window are shown in Table 2. Here, the only marginally statistically significant effect is the reduced P2 amplitude in the Foreign condition (p = 0.05). The results did not markedly change when the model was pruned by removing the Trial regressor.
|Name .||Estimate .||SE .||t .||p .||Lower 95% CI .||Upper 95% CI .|
|Name .||Estimate .||SE .||t .||p .||Lower 95% CI .||Upper 95% CI .|
The results regarding the LSW time window are shown in Table 3. As indicated by the main effect of Speak condition, the estimated SIS was 0.67 μV in the Native condition (p = 0.05). In the Foreign condition, the LSW amplitude for passively heard phonemes was slightly larger (0.26 μV) when compared to the Native condition (p = 0.04). The SIS was 0.43 μV larger in the Foreign condition than in the Native condition (Speak:Foreign, p = 0.02). Finally, the Trial:Speak:Foreign interaction (p = 0.01) suggests that the difference in the SIS between Foreign and Native conditions changed throughout the experiment. The Trial:Speak:Foreign interaction coefficient indicates that, as trial number increased one z unit (roughly 30 trials), the amplitude of the Speak:Foreign interaction increased by 0.49 μV.
|Name .||Estimate .||SE .||t .||p .||Lower 95% CI .||Upper 95% CI .|
|Name .||Estimate .||SE .||t .||p .||Lower 95% CI .||Upper 95% CI .|
Figure 4 visualizes the modeled LWS amplitude and the resulting SIS at three different phases of the experiment. For native phonemes (blue lines), the LSW amplitude, and consequently the SIS, stayed approximately constant throughout the experiment. In contrast, the LSW evoked by the foreign phonemes (orange lines) changed across the trials: Whereas in the Listen condition the LSW amplitudes became more negative, in the Speak condition they became more positive. As a result, the SIS increased throughout the experiment (from 0.28 μV to 1.91 μV).
Correlation Between Pronunciation Ratings and ERPs
The results of the analyses performed so far indicate that foreign phonemes modulate LSW amplitudes and that this effect changes as a function of trials. To test if these changes correlate with improvements in the pronunciation of the foreign phoneme, we performed mixed-effects regression analyses to examine if differences in N1, P2, and LSW amplitudes were predicted by the ratings of foreign phoneme pronunciation accuracy (Rating). The model also included the Speak factor (Speak vs. Listen condition), and the interaction between Speak and Rating. The grand-average ERPs are presented in Figure 5A. In the N1 time window (Figure 5B), the main effect of Rating (p = 0.08) and Speak:Rating interaction (p = 0.27) indicated that N1 amplitudes were not modulated by the accuracy of pronunciation. When the N1 amplitude in the Speak condition was calculated based on an earlier time window (84–140 ms), the main effect of Rating was statistically significant (p = 0.03; Speak:Rating interaction p = 0.27), suggesting that higher ratings were associated with enhanced N1 amplitudes in both Speak and Listen conditions. P2 amplitudes (Figure 5C) did not change as a function of trial in the Listen condition (main effect of Rating, p = 0.63), but P2 was enhanced for phonemes with higher ratings (Speak:Rating, p = 0.014). As shown in Figure 5D, a similar pattern was observed in the LSW time window (main effect of Rating: p = 0.68; Speak:Rating interaction: p = 0.048).
Analysis of Cue Sounds
We analyzed the ERPs produced by the Cue stimuli using linear mixed-effects regression models. The analysis included a factor indicating Condition (Native vs. Foreign phoneme), running (z scored) trial number, and their interaction. This analysis tests to what extent differences in the Listen and Speak conditions can be observed for stimuli not pronounced by the participants. In the N1 time window, foreign phonemes produced stronger ERPs (β = −0.25, t = −1.92, p = 0.054), and this effect increased through the experiment (β = −0.20, t = −2.02, p = 0.042). Models for P2 and LSW amplitudes did not reveal any statistically significant effects.
When learning to pronounce a novel foreign phoneme, the individual needs to acquire novel speech targets based on auditory feedback and by trial and error. We examined whether a well-known marker of auditory feedback control—SIS of auditory evoked activity—is modulated when participants pronounce foreign vs. native phonemes. Specifically, we observed that activity in a relatively late time window (LSW, about 300 ms after vocalization onset) was modulated by the pronunciation of foreign phonemes. This effect is interesting because only LSW amplitudes evoked by self-produced foreign phonemes (but not passively heard foreign phonemes) changed over the course of the experiment, and this effect correlated positively with pronunciation accuracy. When participants vocalized native phonemes, the LSW remained constant throughout the experiment. Because the change in the LSW response parallels improvements in pronunciation, and the effect was specific to pronouncing foreign phonemes, we suggest this result reflects neural processes that mediate the acquisition of novel phonemes.
In line with a large body of research (Behroozmand et al., 2011; Behroozmand & Larson, 2011; Curio et al., 2000; Heinks-Maldonado et al., 2005; Houde et al., 2002; Knolle et al., 2019; Niziolek et al., 2013), we observed a strong SIS. The question arises as to why we did not observe differences between Foreign and Native conditions in SIS in the classical N1 and P2 time windows. Behavioral results indicated that participants had difficulties in pronouncing the foreign /õ/. This suggests that participants did not yet have a clearly defined acoustic or motor target for the foreign phoneme, and their phonation was likely largely based on native phonemes (consistent with this, the formants of the foreign phoneme were biased toward the native /ö/ phoneme). A difference in SIS is not expected if the pronunciation of foreign phoneme relies on similar motor programs as that of native phonemes. The lack of differences in SIS between foreign and native phonemes should not therefore be taken as evidence that the mismatch between desired and produced speech does not modulate SIS. Many previous studies have shown that when vocalization does not match the target, SIS is reduced in amplitude (Behroozmand et al., 2009; Behroozmand & Larson, 2011; Chang et al., 2013; Niziolek et al., 2013). Moreover, the present findings showed that better accuracy in pronouncing foreign phonemes correlated positively with P2 amplitudes in the Speak condition. This indicates that better foreign pronunciation was associated with reduced SIS in the P2 time window.
The fact that we did not find any differences in SIS in the early time windows suggests that learning to better pronounce the foreign phoneme was not strongly associated with low-level auditory feedback control mechanisms. Instead, the change in pronunciation accuracy correlated with LSW amplitudes. When the participants passively listened to the foreign phoneme, the LSW amplitude became more negative across the experiment. This result parallels the findings reported by Alain et al. (2007) and Reinke et al. (2003), who observed that phoneme discrimination training was associated with a modulated LSW. But when the participants in the present study actively pronounced the foreign phoneme, the LSW amplitude became more positive across trials. Consequently, the SIS evoked by foreign phonemes increased as a function of trials. Control analyses of the Cue sounds (/ö/ and /õ/ phonemes spoken by a native speaker) indicated that the difference in the LSW amplitudes cannot be attributed to acoustic differences between the /ö/ and /õ/ phonemes.
What cognitive or behavioral processes does the change in the LSW amplitude to self-produced foreign phonemes reflect? First, because the pronunciation of foreign phonemes improved across the experiment, the LSW amplitude does not appear to reflect fatigue or inattentiveness. Moreover, if the change in LSW reflects fatigue, LSW should be negatively associated with pronunciation accuracy, which is the opposite of what we observed. Second, it could be suggested that the LSW evoked by self-produced phonemes is an error signal, indicating a mismatch between the produced and attempted vocalization. However, if LSW reflects an error signal, it should correlate negatively with pronunciation accuracy. Instead, in the present study, enhanced amplitude of SIS in the LSW time window indicated improved pronunciation. These findings suggest that the LSW may reflect processing of successful vocalizations, which enables the participant to notice and adjust future vocalizations.
The positive amplitude shift in LSW coincides with the timing and topography of the P3a wave, which reflects attentional capture of salient or motivationally relevant stimuli (Knolle et al., 2019; Polich, 2007). The P3a wave is elicited by sensory stimuli that require a behavioral response form the participant (e.g., participant needs to classify a stimulus and respond using a button press; Pitts et al., 2012; Scheerer & Jones, 2018), which likely explains why a P3a wave was not observed in the present study. Although we did not observe a P3a wave per se, the neurocognitive basis of the positive shift in the LSW amplitude may be similar to the mechanisms producing the P3a.
Consciously noticing successful vocalizations is likely a key part of learning to pronounce foreign phonemes because the process requires identifying new acoustic speech targets; it is difficult to improve one’s vocalization of foreign phonemes unless one consciously knows what to aim for. Noticing successful pronunciations could also be interpreted as motivationally relevant reinforcement signals that drive learning. Like the LSW effect we observed, correctly performed actions and feedback after a successful performance are associated with a positive amplitude shift in frontal-central locations (called the “reward positivity”), often interpreted as a correlate of reinforcement signals (Carlson et al., 2011; Glazer et al., 2018; Holroyd & Coles, 2002; Hoy et al., 2021; Ullsperger et al., 2014). Consistent with this, individuals learn better from feedback following successful trials (rather than errors; Arbel et al., 2013; Chiviacowsky & Wulf, 2007). Although, in the present study, the participants were not provided direct feedback on the success of their pronunciation, the playback of their own pronunciation likely functioned as type of feedback signal (Parrell, 2021). Reinforcement learning likely plays a role in learning to produce foreign phonemes but the topic remains little studied (Parrell, 2021).
Based on the similarities to P3a and reward positivity, we suggest that the positive shift in the LSW amplitude to self-produced foreign phonemes may reflect motivational, saliency, or reinforcement signals. These enable the individual to notice successful pronunciations, which then translates to improved pronunciation during the experiment. By successful pronunciation we do not simply refer to the absence of vocalization errors, but to pronunciation that finds its target surprisingly well. These successful pronunciations could be “planned” (i.e., in the sense that this was what the individual attempted to do), but they could also be due to pronunciation errors that, by coincidence, match the target sound better than intended.
Assuming that the sources that contribute to P3a also contribute to LSW, the positive shift in the LSW amplitude could also reflect participants’ conscious confidence in their performance. Frömer et al. (2021) showed that P3a amplitudes correlated with participants’ confidence on the success of their actions. The authors suggest that confidence approximates the amount of noise in the efference copy signals (i.e., their precision), which together with the efference copy and feedback, allow the individuals to make inferences about the success of their action. Their results showed that participants with more accurately “calibrated” confidence (i.e., more accurate estimate of noise in efference copies) learned better (Frömer et al., 2021). This suggests that LSW could reflect higher-order monitoring mechanisms: Whereas SIS in the N1 time window may reflect automatic “online” corrections based on the efference copy (Hickok et al., 2011; Houde & Nagarajan, 2011; Tourville & Guenther, 2011), the LSW may reflect monitoring processes that integrate multiple sources of information (e.g., auditory feedback on one’s pronunciation, predictions based on the efference copy, and the individual’s confidence in the accuracy of these predictions).
In conclusion, our results show that the ability to correctly pronounce a novel foreign phoneme correlates with the amplitude of a late slow ERP (320–440 ms after vocalization). Activity during this time window was differently modulated when participants pronounced foreign (as compared to native) phonemes, and the effect changed during the experiment, paralleling improvements in pronunciation. We propose that activity in the LSW time window reflects high-level performance monitoring processes that signal successful pronunciations and help learn motor commands that produce novel acoustic speech targets. If our interpretation is correct, we expect that the effect in the LSW time window (difference between Foreign and Native conditions) disappears once the individual has successfully learned to produce the novel phoneme (i.e., there is little room, or need for further improvement), and the pronunciation of the foreign phoneme has automatized. A better understanding of the processes that mediate the acquisition of novel phonemes may, in the future, help shed light on various speech-related phenomena, such as the neural processes underlying native language acquisition, second language learning, and rehabilitation of speech deficits.
We thank Ita Puusepp for help with the ratings. We thank Teemu Laine for help with the experimental setup and equipment.
Minna Lehtonen, Research Council of Norway, Award ID: 223265. Pilleriin Sikka, Alfred Österlunds Stiftelse (https://dx.doi.org/10.13039/501100005390). Pilleriin Sikka, Emil Aaltonen Foundation research grant.
Henry Railo: Conceptualization; Formal analysis; Methodology; Supervision; Writing—original draft; Writing—review and editing. Anni Varjonen: Formal analysis; Investigation; Writing—original draft. Minna Lehtonen: Methodology; Writing—review & editing. Pilleriin Sikka: Methodology; Writing—review & editing.
DATA AVAILABILITY STATEMENT
The data, preprocessing, and analysis scripts are available at https://osf.io/wnd2j/.
A distinct sound unit that can distinguish one word from another in a particular language.
- Electroencephalography (EEG):
A method for recording brain-related and other electrical activity from the scalp.
- Event-related potential (ERP):
EEG response produced by a specific event, such as a sound.
- Late slow wave (LSW):
A specific ERP wave with a relatively late timing (>300 ms after stimulus onset).
- Speaking-induced suppression (SIS):
Attenuation of neural responses to self-vocalized sounds relative to passively heard sounds.
Broad peak in the frequency spectrum of a speech sound.
Competing interests: The authors have declared that no competing interests exist.
Handling Editor: Patrick Wong