We present D ef IE, an approach to large-scale Information Extraction (IE) based on a syntactic-semantic analysis of textual definitions. Given a large corpus of definitions we leverage syntactic dependencies to reduce data sparsity, then disambiguate the arguments and content words of the relation strings, and finally exploit the resulting information to organize the acquired relations hierarchically. The output of D ef IE is a high-quality knowledge base consisting of several million automatically acquired semantic relations.